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Table 4 Ingredient composition of the diets and calculated concentrations

From: Impact of coccidiostat and phytase supplementation on gut microbiota composition and phytate degradation in broiler chickens

Ingredient, g/kg

Phase 1 (d 1-10)

Phase 2 (d 10-24/25)

P/Ca+1

P/Ca-1

P/Ca+1

Corn

550

575

575

Soybean Meal

370

350

350

Soy Crude Oil

30

30

30

Monocalcium Phosphate

17

-

11

Limestone

16

11

16

Sand

-

16

-

Vitamin Premix2

2

2

2

Mineral Premix3

0.5

0.5

0.5

DL-Methionine

2

3.5

3.5

Sodium Bicarbonate

3

3

3

Sodium Chloride

1.5

1

1

Choline Chloride

2

2

2

TiO2

5

5

5

Calculated composition, g/kg DM

 Crude Protein

237

230

230

 Total Phosphorus (tP)

8.5

4.2

7.0

 Calcium

11.5

6.5

10.4

 Ca:tP

1.3

1.5

1.5

  1. 1Includes treatments Phy-Coc-, Phy-Coc+, Phy+Coc-, and Phy+Coc+, where Phy- = 0 and Phy+ = 1500 FTU phytase/kg, Coc- = 0 and Coc+ = 50 mg/kg of Narasin and Nicarbazin each in exchange for sand
  2. 2Vitamin premix (Miavit GmbH, Essen, Germany), provided per kg of complete diet: 10 000 IU vitamin A, 3000 IU vitamin D3, 30 mg vitamin E, 2.4 mg vitamin K3, 100 mcg biotin, 1 mg folic acid, 3 mg vitamin B1, 6 mg vitamin B2, 6 mg vitamin B6, 30 mcg vitamin B12, 50 mg nicotinamide, 14 mg calcium-D-pantothenat
  3. 3Trace element premix (Gelamin Gesellschaft für Tierernährung mbH, Memmingen, Germany), provided per kg of complete diet: 25 mg calcium from carbonate, 80 mg manganese from manganese-(II)-oxide, 60 mg zinc from zinc-oxide, 25 mg iron from ferrous-(II)-sulphate monohydrate, 7.5 mg copper from cupric-(II)-sulphate pentahydrate, 0.6 mg iodine from calcium iodate, 0.2 mg selenium from sodium selenite