TY - JOUR AU - Hartman, Leon Michael AU - van Oppen, Madeleine Josephine Henriette AU - Blackall, Linda Louise PY - 2020 DA - 2020/04/05 TI - Microbiota characterization of Exaiptasia diaphana from the Great Barrier Reef JO - Animal Microbiome SP - 10 VL - 2 IS - 1 AB - Coral reefs have sustained damage of increasing scale and frequency due to climate change, thereby intensifying the need to elucidate corals’ biological characteristics, including their thermal tolerance and microbial symbioses. The sea anemone, Exaiptasia diaphana, has proven an ideal coral model for many studies due to its close phylogenetic relationship and shared traits, such as symbiosis with algae of the family Symbiodiniaceae. However, established E. diaphana clonal lines are not available in Australia thus limiting the ability of Australian scientists to conduct research with this model. To help address this, the bacterial and Symbiodiniaceae associates of four Great Barrier Reef (GBR)-sourced E. diaphana genotypes established in laboratory aquaria and designated AIMS1–4, and from proxies of wild GBR E. diaphana were identified by metabarcoding of the bacterial 16S rRNA gene and eukaryotic rRNA gene ITS2 region. The relationship between AIMS1–4 and their bacterial associates was investigated, as was bacterial community phenotypic potential. Existing data from two existing anemone clonal lines, CC7 and H2, were included for comparison. SN - 2524-4671 UR - https://doi.org/10.1186/s42523-020-00029-5 DO - 10.1186/s42523-020-00029-5 ID - Hartman2020 ER -