Microbial environment shapes immune function and cloacal microbiota dynamics in zebra finches Taeniopygia guttata

Table 2 Repeatability of innate immune indices and cloacal microbiota characteristics of female zebra finches

Immune index	Treatment	Principal coordinate axis	R	SE	95% CI (lower, upper)^b	P^c
Agglutination (titer)			0.14	0.07	0.017, 0.301	0.033
[IgY] (absorbance)			0.80	0.05	0.65, 0.866	0.001
Lytic activity (probability)^a			0.06	0.20	0, 0.894	0.198
[Haptoglobin] (mg ml⁻¹)			0.26	0.08	0.113, 0.423	0.001
Multivariate immune function	High diversity	axis 1	0.37	0.13	0.082, 0.584	0.004
		axis 2	0.56	0.13	0.265, 0.75	0.001
	Low diversity	axis 1	0.08	0.11	0, 0.333	0.242
		axis 2	0.74	0.11	0.437, 0.85	0.001
Cloacal microbiota
OTU richness			0.18	0.10	0.005, 0.394	0.019
Shannon’s diversity			0.23	0.09	0.064, 0.421	0.005
Cloacal taxon occurrence (unweighted UniFrac)	High diversity	axis 1	0.00	0.07	0, 0.223	1.000
		axis 2	0.46	0.13	0.168, 0.662	0.001
	Low diversity	axis 1	0.00	0.00	0, 0	0.980
		axis 2	0.28	0.14	0, 0.536	0.008

^aNo detected lysis titres at sampling moment 3 and 4 inhibited evaluation of differences among sampling moment categories; a logit link GLMM with continuous temporal predictor was fitted instead
^bConfidence intervals based on 1000 parametric bootstraps
^cP-values calculated based on 1000 permutations

ISSN: 2524-4671