Survey of rumen microbiota of domestic grazing yak during different growth stages revealed novel maturation patterns of four key microbial groups and their dynamic interactions

Guo, Wei; Zhou, Mi; Ma, Tao; Bi, Sisi; Wang, Weiwei; Zhang, Ying; Huang, Xiaodan; Guan, Le Luo; Long, Ruijun

doi:10.1186/s42523-020-00042-8

Animal Microbiome

Table 1 Sample information and sequencing statistics obtained using the DADA2 algorithm in QIIME2

From: Survey of rumen microbiota of domestic grazing yak during different growth stages revealed novel maturation patterns of four key microbial groups and their dynamic interactions

Age group	Number of samples	Number of sequences	Frequency	Number of ESVs	Good's coverage
Bacteria
7d	5	52,238 ± 12,630	30,010 ± 12,440	319 ± 120	99.32% ± 0.003
14d	5	55,016 ± 18,393	16,227 ± 5805	291 ± 144	99.57% ± 0.003
1m	6	60,430 ± 12,638	27,747 ± 7213	394 ± 175	99.23% ± 0.004
2m	5	52,145 ± 16,144	15,312 ± 4997	697 ± 144	98.90% ± 0.005
3m	6	56,182 ± 16,390	15,824 ± 5856	738 ± 263	98.68% ± 0.008
4m	7	56,350 ± 12,375	16,736 ± 4395	810 ± 193	98.66% ± 0.005
6m	6	59,274 ± 4492	14,909 ± 2458	848 ± 80	98.67% ± 0.003
1y	5	56,981 ± 16,552	14,641 ± 6165	883 ± 234	98.44% ± 0.006
2y	6	56,913 ± 6502	14,646 ± 2829	994 ± 172	98.31% ± 0.005
3y	5	55,345 ± 11,704	13,502 ± 4249	988 ± 316	98.25% ± 0.009
5y	6	50,667 ± 9955	10,604 ± 3126	835 ± 214	98.72% ± 0.005
8y	6	55,476 ± 15,398	12,290 ± 4057	962 ± 277	98.35% ± 0.007
10y	6	60,056 ± 7647	13,461 ± 1935	1074 ± 139	98.14% ± 0.003
12y	6	55,415 ± 12,325	12,414 ± 3633	995 ± 281	98.25% ± 0.006
P value				< 0.0001	> 0.05
Archaea
14d	5	19,372 ± 3328	10,754 ± 3343	44 ± 12	99.94% ± 0.0001
1m	5	16,897 ± 4360	10,402 ± 2831	40 ± 23	99.90% ± 0.0001
2m	5	19,169 ± 6558	9066 ± 2217	28 ± 8	99.99% ± 0.0001
3m	6	14,920 ± 2467	6541 ± 1574	27 ± 7	99.99% ± .0001
4m	7	16,856 ± 3321	7817 ± 1699	38 ± 10	99.98% ± 0.0002
6m	6	13,792 ± 4255	6179 ± 1999	38 ± 5	99.99% ± 0.0001
1y	5	18,556 ± 5254	8282 ± 2695	43 ± 5	99.99% ± 0.00003
2y	6	15,102 ± 5243	7667 ± 2777	47 ± 11	99.97% ± 0.0002
3y	6	16,690 ± 4738	7392 ± 1563	46 ± 4	99.99% ± 0.0001
5y	6	15,866 ± 2104	6553 ± 1827	42 ± 7	99.99% ± 0.0001
8y	6	15,490 ± 994	7309 ± 816	47 ± 3	99.99% ± 0.0001
10y	6	15,643 ± 4837	6672 ± 11	43 ± 11	99.99% ± 0.0002
12y	6	19,596 ± 3692	7472 ± 1577	42 ± 5	99.99% ± 0.0001
P value				0.007	> 0.05
Fungi
1m	5	63,933 ± 12,105	43,217 ± 17,325	26 ± 11	99.79% ± 0.0003
2m	5	63,156 ± 8927	43,383 ± 13,052	49 ± 18	99.95% ± 0.0004
3m	5	65,614 ± 13,940	42,452 ± 9451	48 ± 16	99.97% ± 0.0001
4m	6	67,611 ± 7412	47,666 ± 7239	46 ± 17	99.96% ± 0.0001
6m	5	69,635 ± 4601	31,080 ± 23,236	41 ± 22	99.95% ± 0.0006
1y	5	63,303 ± 6821	42,651 ± 4103	43 ± 18	99.96% ± 0.0003
2y	6	70,881 ± 4236	51,483 ± 2749	57 ± 18	99.94% ± 0.0003
3y	6	64,915 ± 9110	44,740 ± 6529	66 ± 12	99.95% ± 0.0002
5y	5	59,552 ± 16,489	39,604 ± 9599	52 ± 22	99.97% ± 0.0002
8y	6	66,924 ± 3850	45,155 ± 3932	68 ± 25	99.94% ± 0.0006
10y	5	70,885 ± 1638	48,606 ± 2227	70 ± 12	99.93% ± 0.0004
12y	6	66,724 ± 8008	39,244 ± 6064	65 ± 15	99.96% ± 0.0001
P value				0.008	> 0.05
Protozoa
1m	6	22,409 ± 7699	15,927 ± 5932	31 ± 16	99.86% ± 0.0007
2m	5	18,364 ± 1862	12,538 ± 2959	12 ± 5	99.93% ± 0.0003
3m	6	16,334 ± 3107	7184 ± 3070	20 ± 8	99.92% ± 0.0011
4m	7	17,212 ± 4875	7376 ± 3130	24 ± 13	99.91% ± 0.0008
6m	6	20,446 ± 5872	6143 ± 1896	41 ± 15	99.75% ± 0.0017
1y	5	19,728 ± 1913	6910 ± 1912	48 ± 7	99.81% ± 0.0005
2y	6	18,919 ± 6117	7068 ± 2740	65 ± 27	99.51% ± 0.0026
3y	6	15,910 ± 3081	5687 ± 1563	73 ± 11	99.54% ± 0.0009
5y	6	16,860 ± 3405	5547 ± 1405	68 ± 11	99.48% ± 0.0001
8y	6	19,072 ± 7301	6817 ± 3726	91 ± 20	99.36% ± 0.0031
10y	6	16,736 ± 2231	5934 ± 1709	72 ± 11	99.51% ± 0.0010
12y	6	17,061 ± 2908	6826 ± 2536	73 ± 16	99.60% ± 0.0011
P value				< 0.0001	> 0.05

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