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Table 4 The taxonomic classification of core OTUs (> 90% of all samples) based on RDP classification (v.10). The results of repeated measures ANOVA of the relative abundance of each OTU is also included. F statistics are included with degrees of freedom (Fdf, dfDen) where dfDen is the denominator df based on Kenward-Roger first order approximations with Kacker-Harville correction. * p < 0.05, ** p < 0.01, *** p < 0.001. Where significant, partial η2 effect sizes are presented parenthetically

From: Recovery and resiliency of skin microbial communities on the southern leopard frog (Lithobates sphenocephalus) following two biotic disturbances

Phylum (Order)

Family (Genus)

OTU #

Treatment

Time

Treatment x Time

Actinobacteria

 Actinomycetales

Corynebacteriaceae (Corynebacterium)

76

F3,23.2 = 2.364

F5,101.7 = 13.511***(0.354)

F15,100.9 = 1.305

Dermacoccaceae (Dermacoccus)

28

F3,22.3 = 1.183

F5,100.0 = 29.749***(0.547)

F15,99.3 = 2.643**(0.244)

Micrococcaceae (Micrococcus)

31i

F3,23.4 = 1.482

F5,106.2 = 30.343***(0.552)

F15,105.8 = 1.459

Micrococcaceae (Nesterenkonia)

2a

24

F3,18.9 = 0.454

F3,18.9 = 0.409

F5,103.6 = 23.845***(0.492)

F5,100.0 = 23.353***(0.487)

F15,103.2 = 3.206***(0.281)

F15,99.6 = 3.253***(0.284)

Propionibacteriaceae (Propionibacterium)

9

F3,18.9 = 1.270

F5,99.2 = 16.822***(0.406)

F15,98.1 = 3.849***(0.319)

Bacteriodetes

 Flavobacteriales

Flavobacteriaceae (Flavobacterium)

15

81

F3,22.1 = 0.478

F3,17.6 = 0.396

F5,99.5 = 9.886***(0.288)

F5,89.2 = 12.982***(0.345)

F15,99.0 = 1.387

F15,88.6 = 0.877

Firmicutes

 Bacillales

Bacillales incertae sedis (Caldalkalibacillus)

3a

26a

102

F3,22.1 = 1.657

F3,22.3 = 2.954

F3,19.7 = 0.182

F5,107.6 = 20.691***(0.456)

F5,109.3 = 19.829***(0.446)

F5,89.9 = 9.516***(0.279)

F15,107.2 = 2.807***(0.255)

F15,108.8 = 2.068*(0.201)

F15,88.7 = 2.069*(0.201)

Bacillaceae (Bacillus)

29

F3,21.4 = 1.246

F5,10,442 = 16.592***(0.402)

F15,103.9 = 1.998*(0.196)

Bacillaceae (Geobacillus)

100

F3,20.2 = 2.921

F5,93.3 = 13.386***(0.352)

F15,92.6 = 1.267

Proteobacteria

 Burkholderiales

Alcaligenaceae (Bordetella)

1i

F3,22.2 = 0.304

F5,101.9 = 17.454***(0.415)

F15,101.6 = 0.889

Comamonadaceae (Delftia)

16i

F3,25.0 = 0.793

F5,107.8 = 14.084***(0.364)

F15,107.4 = 2.077*(0.202)

Comamonadaceae (Comamonas)

18i

F3,23.2 = 1.510

F5,103.1 = 7.340***(0.229)

F15,102.3 = 1.731

Comamonadaceae (unclassified)

41

F3,22.6 = 1.869

F5,103.7 = 9.564***(0.281)

F15,103.5 = 1.111

 Pseudomonadales

Moraxellaceae (Acinetobacter)

5i

20e

F3,22.1 = 0.498

F3,24.7 = 0.724

F5,105.7 = 11.316***(0.315)

F5,100.7 = 11.135***(0.311)

F15,105.3 = 1.139

F15,100.5 = 1.202

Moraxellaceae (Enhydrobacter)

25

F3,22.6 = 5.52**§(0.118)

F5,109.2 = 12.433***(0.335)

F15,107.7 = 1.397

Pseudomonadaceae (Pseudomonas)

10i

F3,21.6 = 0.634

F5,97.7 = 7.911***(0.243)

F15,96.8 = 0.931

 Oceanospirillales

Halomonadaceae (Halomonas)

17a

F3,23.3 = 1.485

F5,108.9 = 26.467***(0.518)

F15,108.5 = 2.392**(0.225)

 Enterobacteriales

Enterobacteriaceae (Cronobacter)

21i

F3,21.9 = 0.623

F5,102.2 = 12.679***(0.340)

F15,101.8 = 1.091

 Methylophilales

Methylophilaceae (Methylophilus)

49

F3,20.7 = 2.291

F5,94.0 = 5.107***(0.172)

F15,93.4 = 1.047

 Sphingomonadales

Sphingomonadaceae (Sphingomonas)

52

F3,22.3 = 1.350

F5,99.8 = 18.892***(0.434)

F15,99.3 = 1.227

  1. a = OTUs present in all frogs at all time points
  2. i = OTUs with 399% sequence similarity for Bd-inhibiting bacteria (Woodhams et al. 2015)
  3. e = OTUs with 399% sequence similarity for Bd-enhancing bacteria (Woodhams et al. 2015)
  4. §=Treatment effect - Tukey HSD indicates Bd (A), BB (AB), N (B), Btk (B) where A > B