Table 2 Strains and plasmids used in this study

S. Typhimurium ST4/74

Nal^R

[30]

ST4/74 ΔinvA

Non-polar invA deletion mutant

This study

ST4/74 ΔSTM3254

Non-polar STM3254 deletion mutant with zero growth phenotype in galactitol

[29]

ST4/74 ΔSTM3254::KanR

Mutant with allelic exchange of STM3254 against a kanamycin resistance gene

This study

ST4/74 ΔgatR-HTH

Partial non-polar deletion of gatR lacking the nucleotides encoding the helix-turn-helix motif (HTH)

This study

E. coli

DH5α

F^– endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG purB20 φ80ΔlacZΔM15 Δ(lacZYA-argF )U169, hsdR17 (r_K^–m_K⁺), λ^–

Plasmids

pBR322

Amp^R, Tet^R

[31]

pBR-STM3254

pBR322 with gene STM3254

This study

pBR-gatR

pBR322 with repressor gene gatR

This study

pKD4

Kan^R, pir-dependent, FRT sites

[32]

pKD46

λ-Red helper plasmid, Amp^R

[32]

pCP20

FLP recombinase plasmid, Cm^R, Amp^R

[32]