Metatranscriptomic analyses reveal ruminal pH regulates fiber degradation and fermentation by shifting the microbial community and gene expression of carbohydrate-active enzymes

Table 1 Functional pathways that were significantly different among treatments^a

Pathway	Control		LpH		SEM	P value
Pathway	Liquid	Solid	Liquid	Solid	SEM	Con vs LpH^b	Con vs LpH^c	Liquid vs Solid^d
GLYCOLYSIS: glycolysis I (from glucose 6-phosphate)	0.25	0.33	0.09	0.28	0.07	0.05	0.53	0.03
PWY-5484: glycolysis II (from fructose 6-phosphate)	0.23	0.28	0.08	0.22	0.07	0.04	0.43	0.06
ILEUSYN-PWY: L-isoleucine biosynthesis I (from threonine)	0.12	0.27	0.07	0.24	0.05	0.50	0.60	0.002
PWY-5100: pyruvate fermentation to acetate and lactate	0.20	0.25	0.06	0.23	0.06	0.04	0.72	0.03
P108-PWY: pyruvate fermentation to propanoate	0.67	0.56	0.25	0.49	0.12	0.03	0.69	0.60

^aRelative abundances of functional pathways were transformed to centered log ratio to avoid compositional data problem
^bControl versus LpH within the ruminal liquid fraction
^cControl versus LpH within the ruminal solid fraction
^dRuminal sample fraction effect regardless of ruminal pH

ISSN: 2524-4671