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Table 1 Substrates used for in vitro fermentations with freshly weaned piglet colon content

From: High throughput in vitro characterization of pectins for pig(let) nutrition

Substrates

DE%

DAc%

MW, DP

Sugar composition (mol%)

IV (dL/g)

Ferulic acid (µmol/g DM)

Supplier

1. Sugar Beet Pulp (SBPulp) (dried, 1 mm particle size)

n.d

n.d

I

GalA: 31.2, Rha: 2.7, Ara: 26.9, Gal: 7.0, other:32.2

n.d

41.7 ± 1.6

CP Kelco

2. Sugar Beet Pectin (SBP)*

52

21

69 kDa

GalA: 71.9, Rha: 4.8, Ara: 6.6, Gal: 13.5, other:3.1

2.8

50.5 ± 3.0

CP Kelco

3. RG-I Sugar Beet (SBP-RG-I)*

      

CP Kelco

4. Sugar Beet LM 1B, Random (LM1B)

44.8

20.1

175 kDa

GalA: 73

2.6

n.d

CP Kelco

5. Sugar Beet LM 1C, Random (LM1C)

37.8

20.3

183 kDa

GalA: 71

2.5

n.d

CP Kelco

6. Sugar Beet LM 1D, Random (LM1D)

22.9

20.4

101 kDa

GalA: 69

2.2

n.d

CP Kelco

7. Sugar Beet LM 2, Blocky (LM2)

35.1

20.5

54 kDa

GalA: 69

1.4

n.d

CP Kelco

8. Sugar Beet LM 2A, Blocky (LM2A)

44.4

20.5

124 kDa

GalA: 73

2.1

n.d

CP Kelco

9. Arabino-oligosaccharides (ModAr)

n.d

n.d

DP 2-15

GalA: 10.4, Rha: 2.5, Ara: 75.1, Gal: 9.6, other:2.4

n.d

52.5 ± 2.9

Holck 2011

10. Arabinan (Ar) from Sugar Beet

n.d

n.d

49 kDa

GalA: 10.2, Rha: 1.4, Ara: 69.0, Gal: 18.7, other:0.7

n.d

n.d

Megazyme

11. Debranched Arabinan (DebAr)

n.d

n.d

28 kDa

GalA: 6.0, Rha: 2.0, Ara: 88, Gal: 4

n.d

n.d

Megazyme

12. Polygalacturonic Acid (C-PG)

n.d

n.d

PI, 13 kDa

 

n.d

n.d

Sigma Aldrich

13. JEH modified Pectin from Sugar Beet: enriched RGI fraction from sugar beet (ModSBP)

n.d

n.d

50 kDa

GalA: 49.4, Rha: 7.5, Ara: 11.7, Gal: 16.5, other:15.0

n.d

n.d

Holck 2011 tailored

14. Citrus HMP (C-HMP)*

       

15. RG-I Citrus (C-RG-I)*

n.d

n.d

  

n.d

n.d

CP Kelco

16. Inulin from chicory (Inulin)

n.d

n.d

PI, 2 kDa

 

n.d

n.d

Sigma Aldrich

17. Fructo-oligosaccharides (FOS) (Orafti P95; Oreye, Belgium)

n.d

n.d

DP 2-13

 

n.d

n.d

Beneo-Orafti

18. Galacto-oligosaccharides (GOS)

n.d

n.d

DP 2–8

 

n.d

n.d

Beneo-Orafti

19. Pea Pectin (PP)

n.d

n.d

  

n.d

n.d

CP Kelco

20. Arabinogalactan (AG)

n.d

n.d

  

n.d

n.d

Sigma-Aldrich

  1. All fermentations tested with 1% (w/v) substrate except were indicated with *, where 0.5% (w/v) was also tested
  2. Molecular structure details such as degree of esterification (DE), degree of acetylation (DAc), molecular weight (MW), degree of polymerization (DP), intrinsic viscosity (IV) and the sugar composition is also listed (abbreviations: n.d. not determined, LM low methylated, HMP high methylated citrus pectin, RG rhamnogalacturonan, GalA galacturonic acid, Rha rhamnose, Ara arabinose, Gal galactose, S soluble, IN insoluble, PI partly insoluble)