The rumen liquid metatranscriptome of post-weaned dairy calves differed by pre-weaning ruminal administration of differentially-enriched, rumen-derived inocula

Table 4 Differentially abundant CAZymes by microbial inoculations

Dominance	CAZymes	Relative abundance (%)^*		SEM	P-value	Known activities
Dominance	CAZymes	BE(+)	BE(−)	SEM	P-value	Known activities
BE(+)	GH19	0.010	0	0.003	0.031	chitinase (EC 3.2.1.14)
	GT84	0.053	0.004	0.013	0.009	cyclic β-1,2-glucan synthase (EC 2.4.1.-)
	PL14	0.067	0.013	0.011	0.010	alginate lyase (EC 4.2.2.3)
BE(−)	CE14	0.195	0.310	0.032	0.041	N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-α-D-glucopyranoside deacetylase
BE(−)	GH89	0.147	0.242	0.026	0.023	α-N-acetylglucosaminidase

Dominance	CAZymes	Relative abundance (%)		SEM	P-value	Known activities
Dominance	CAZymes	PE(+)	PE(−)	SEM	P-value	Known activities
PE(+)	GH16	0.314	0.182	0.038	0.041	xyloglucan:xyloglucosyltransferase (EC 2.4.1.207)
	GH39	0.363	0.253	0.056	0.028	β-xylosidase (EC 3.2.1.37)
	GH142	0.125	0.054	0.017	0.023	β-L-arabinofuranosidase (EC 3.2.1.185)
	PL10	0.121	0.032	0.017	0.006	pectate lyase (EC 4.2.2.2)
	PL33	0.051	0.015	0.008	0.006	hyaluronate lyase (EC 4.2.2.1)
PE(−)	CBM2	0.011	0.098	0.019	0.033	cellulose-binding domain
	CE11	0.662	0.991	0.061	0.007	UDP-3–0-acyl N-acetylglucosamine deacetylase (EC 3.5.1.108)
	GH92	0.574	0.959	0.082	0.010	mannosyl-oligosaccharide α-1,2-mannosidase (EC 3.2.1.113)
	GH130	0.416	0.864	0.084	0.008	β-1,4-mannosylglucose phosphorylase (EC 2.4.1.281)
	GH133	0.861	1.161	0.067	0.023	amylo-α-1,6-glucosidase (EC 3.2.1.33)
	GT4	2.064	2.602	0.097	0.005	sucrose synthase (EC 2.4.1.13)

Only the CAZymes which have over 0.01% average relative abundance in at least one of the treatments [BE(+), BE(−), PE(+), PE(−)] were statistically analyzed by LEfSe
BE, bacterial-enriched rumen fluid; PE, protozoal-enriched rumen fluid
*Calculated based on the normalized CAZymes count tables

ISSN: 2524-4671