Fig. 5

Threshold Indicator Taxa Analysis (TITAN) for bacterial taxa in response to prolactin concentrations. A We identified bacterial changepoints in association with prolactin (PRL) concentrations, with a collective positive changepoint identified at 17.7 ng/ml. Circles represent bacterial taxa changepoints with red circles representing a positive response to prolactin (z+) and blue circles representing a negative (z−) response to prolactin. The size of the circle reflects the magnitude of the Indicator Species Analysis statistic for the bacterial ASV. Horizontal lines represent the 5th and 95th percentiles among 500 bootstrap replicates. Examples of bacterial taxon abundance and density with a B negative response and C positive response to increasing prolactin concentrations. Blue lines represent bootstrap replicates for identifying change point. Red line indicates change point