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Table 1 Representation of sampling design and depiction of the number of samples (n) collected for each category

From: Early life skin microbial trajectory as a function of vertical and environmental transmission in Bornean foam-nesting frogs

Species Nest Body site for each adult (male/female pair per species) Tadpoles from nest Environmental samples of nest Tadpoles from pond Environmental samples of pond
Interior Exterior Dorsal Ventral Cloaca Leaf Water (terrarium) Water (pond)
Polypedates leucomystax 3* 3* 2 2 2 3 3* 3 3 3
P. macrotis 3* 3* 2 2 2 3 3* 3 3 3
P. otilophus 3* 3* 2 2 2 3 3* 3 3 3
Total 9 9 6 6 6 9 9 9 9 9
  1. We collected three samples within each nest from different areas of the nest interior and exterior as well as different areas on the leaf to which each nest was attached (denoted by an asterisk) and considered them independent samples as they represented distinct nest or leaf microhabitats. Each nest sampled consists of a pair of adults, nest, environment, and tadpoles both before and after environmental interaction (Graphical representation of sampling design, Fig. 1)