Application of 3-nitrooxypropanol and canola oil to mitigate enteric methane emissions of beef cattle results in distinctly different effects on the rumen microbial community

Table 1 The impact of feeding diets supplemented with (+) and without (−) 3-NOP and OIL on the α-diversity of the rumen microbial community at 0, 6 and 12 h after feeding in beef cattle

Item	−3-NOP		+3-NOP		SEM	P value
Item	−OIL	+OIL	−OIL	+OIL	SEM	3-NOP	OIL	3-NOP × OIL
Rumen fluid
0 h
Observed ASVs	560	406	501	383	23.7	0.095	< 0.001	0.46
Phylogenetic diversity	45.5	34.9	41.5	33.4	0.72	0.014	< 0.001	0.25
6 h
Observed ASVs	536	379	457	335	28.8	0.021	< 0.001	0.49
Phylogenetic diversity	43.8	33.7	38.2	30.8	1.23	0.001	< 0.001	0.24
12 h
Observed ASVs	528	393	429	344	32.1	0.002	< 0.001	0.27
Phylogenetic diversity	43.0	34.1	37.7	31.4	1.28	0.013	0.001	0.37
Rumen Digesta
0 h
Observed ASVs	820	614	738	657	32.3	0.55	< 0.001	0.062
Phylogenetic diversity	54.8^a	42.6^c	50.3^b	43.6^c	1.47	0.14	< 0.001	0.024
6 h
Observed ASVs	747	592	696	603	17.7	0.46	< 0.001	0.26
Phylogenetic diversity	50.3	42.4	48.2	42.1	1.69	0.37	< 0.001	0.49
12 h
Observed ASVs	810	604	686	582	7.1	0.017	< 0.001	0.084
Phylogenetic diversity	52.5	42.1	47.5	40.3	2.44	0.006	< 0.001	0.14

ISSN: 2524-4671