Application of 3-nitrooxypropanol and canola oil to mitigate enteric methane emissions of beef cattle results in distinctly different effects on the rumen microbial community

Gruninger, Robert J.; Zhang, Xiu Min; Smith, Megan L.; Kung, Limin; Vyas, Diwakar; McGinn, Sean M.; Kindermann, Maik; Wang, Min; Tan, Zhi Liang; Beauchemin, Karen A.

doi:10.1186/s42523-022-00179-8

Animal Microbiome

Table 2 Impact of feeding diets supplemented with (+) and without (−) 3-NOP and OIL on the relative abundance of phyla identified in rumen fluid in beef cattle

From: Application of 3-nitrooxypropanol and canola oil to mitigate enteric methane emissions of beef cattle results in distinctly different effects on the rumen microbial community

Item	-3-NOP		+ 3-NOP		SEM	P value
Item	-OIL	+ OIL	-OIL	+ OIL	SEM	3-NOP	OIL	3-NOP × OIL
0 h
Euryarchaeota	1.18	0.71	0.62	0.21	0.165	< 0.001	0.002	0.80
Bacteroidetes	54.9^b	59.7^ab	57.4^ab	62.7^a	2.75	0.11	0.007	0.023
Firmicutes	19.3	21.8	18.6	21.2	3.03	0.60	0.052	0.93
Fibrobacteres	10.6	0.26	10.2	0.14	1.16	0.81	< 0.001	0.89
Actinobacteria	0.40	0.15	0.54	0.14	0.158	0.67	0.038	0.62
Proteobacteria	4.18	12.0	3.48	12.0	1.55	0.81	< 0.001	0.84
Spirochaetae	1.97	2.36	3.48	1.89	0.532	0.31	0.24	0.061
Verrucomicrobia	4.10	1.24	3.65	0.82	0.586	0.30	< 0.001	0.97
Others (< 0.5%)	3.31	1.79	2.09	0.94	0.341	< 0.001	< 0.001	0.43
F: B	0.32	0.34	0.29	0.31	0.009	0.17	0.43	0.95
6 h
Euryarchaeota	0.89^a	0.54^b	0.17^c	0.11^c	0.072	< 0.001	0.002	0.016
Bacteroidetes	45.0	49.5	53.1	49.9	2.77	0.042	0.75	0.059
Firmicutes	25.5	24.6	18.3	25.1	2.92	0.21	0.27	0.15
Fibrobacteres	7.68	0.06	6.04	0.02	1.018	0.39	< 0.001	0.42
Actinobacteria	2.15	0.22	0.98	0.39	0.553	0.34	0.022	0.20
Proteobacteria	12.5	22.1	15.2	23.1	3.79	0.58	0.016	0.79
Spirochaetae	2.36	1.25	3.66	0.81	0.538	0.43	0.001	0.12
Verrucomicrobia	2.22	0.66	1.64	0.27	0.237	0.019	< 0.001	0.64
Others (< 0.5%)	1.72	1.02	0.89	0.34	0.192	< 0.001	< 0.001	0.54
F: B	0.50	0.44	0.28	0.43	0.040	0.076	0.28	0.086
12 h
Euryarchaeota	0.85	0.63	0.26	0.12	0.086	< 0.001	0.003	0.47
Bacteroidetes	50.8	49.3	52.0	55.6	2.24	0.070	0.60	0.20
Firmicutes	17.3	20.4	13.6	19.5	2.20	0.21	0.023	0.46
Fibrobacteres	11.7	0.16	11.3	0.06	1.37	0.87	< 0.001	0.93
Actinobacteria	0.86	0.28	0.63	0.32	0.252	0.66	0.053	0.55
Proteobacteria	11.2	25.8	15.4	22.3	3.06	0.92	0.002	0.21
Spirochaetae	2.40	1.31	3.75	1.19	0.564	0.21	0.001	0.14
Verrucomicrobia	2.61	0.83	1.96	0.44	0.286	0.041	< 0.001	0.59
Others (< 0.5%)	2.26	1.19	1.08	0.50	0.188	< 0.001	< 0.001	0.21
F: B	0.32	0.38	0.23	0.31	0.026	0.037	0.042	0.39

OIL, canola oil; 3-NOP, 3-nitrooxypropanol; F: B, Firmicutes: Bacteroidetes
^a,b,cValues within a row with different letters differ (P ≤ 0.05)

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ISSN: 2524-4671

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