Application of 3-nitrooxypropanol and canola oil to mitigate enteric methane emissions of beef cattle results in distinctly different effects on the rumen microbial community

Gruninger, Robert J.; Zhang, Xiu Min; Smith, Megan L.; Kung, Limin; Vyas, Diwakar; McGinn, Sean M.; Kindermann, Maik; Wang, Min; Tan, Zhi Liang; Beauchemin, Karen A.

doi:10.1186/s42523-022-00179-8

Animal Microbiome

Table 3 Impact of feeding diets supplemented with (+) and without (−) 3-NOP and OIL on the relative abundance of phyla identified in rumen digesta in beef cattle at 0, 6 and 12 h after feeding

From: Application of 3-nitrooxypropanol and canola oil to mitigate enteric methane emissions of beef cattle results in distinctly different effects on the rumen microbial community

Item	−3-NOP		+3-NOP		SEM	P value
Item	−OIL	+OIL	−OIL	+OIL	SEM	3-NOP	OIL	3-NOP × OIL
0 h
Euryarchaeota	3.43^b	6.04^a	2.18^b	1.69^b	0.631	< 0.001	0.031	0.003
Bacteroidetes	24.4	36.9	30.0	39.0	1.56	0.005	< 0.001	0.15
Firmicutes	48.4	51.3	45.0	52.7	1.55	0.49	0.001	0.096
Fibrobacteres	14.6	0.06	11.8	0.05	1.45	0.33	< 0.001	0.34
Actinobacteria	1.22	0.74	1.58	1.17	0.368	0.25	0.19	0.91
Proteobacteria	1.15	2.28	0.90	2.53	0.293	0.98	< 0.001	0.40
Spirochaetae	4.43	1.54	6.73	1.81	0.707	0.078	< 0.001	0.16
Verrucomicrobia	0.72	0.29	0.67	0.30	0.080	0.71	< 0.001	0.67
F: B	1.92	1.37	1.47	1.33	0.118	0.026	0.002	0.063
Others (< 0.5%)	1.59	0.85	1.14	0.74	0.171	0.11	0.002	0.32
6 h
Euryarchaeota	3.24^a	4.11^a	1.39^b	0.96^b	0.466	< 0.001	0.44	0.030
Bacteroidetes	23.8	33.2	30.5	35.5	1.34	0.001	< 0.001	0.056
Firmicutes	52.1^a	51.6^ab	46.6^b	51.9^a	1.43	0.052	0.068	0.032
Fibrobacteres	9.97	0.05	9.27	0.03	1.18	0.75	< 0.001	0.77
Actinobacteria	4.00	0.83	2.62	1.49	1.17	0.74	0.061	0.36
Proteobacteria	2.69	8.23	3.95	8.31	1.122	0.52	< 0.001	0.58
Spirochaetae	2.47	1.11	4.46	1.14	0.525	0.065	< 0.001	0.073
Verrucomicrobia	0.53	0.23	0.51	0.19	0.067	0.62	< 0.001	0.90
F: B	2.17^b	1.54^a	1.52^a	1.45^a	0.145	0.002	0.004	0.020
Others (< 0.5%)	1.18	0.60	0.73	0.50	0.170	0.10	0.023	0.29
12 h
Euryarchaeota	3.11^b	4.51^a	1.48^c	0.84^c	0.498	< 0.001	0.25	0.005
Bacteroidetes	26.7	35.9	32.7	39.2	1.20	< 0.001	< 0.001	0.22
Firmicutes	50.0	49.2	44.9	50.1	1.52	0.14	0.13	0.044
Fibrobacteres	9.38	0.09	8.18	0.06	1.052	0.55	< 0.001	0.57
Actinobacteria	2.73	0.69	2.17	1.28	0.848	0.98	0.052	0.43
Proteobacteria	2.90	7.51	4.06	6.71	0.959	0.85	0.001	0.31
Spirochaetae	2.96^b	1.14^c	5.18^a	1.19^c	0.531	0.041	< 0.001	0.051
Verrucomicrobia	0.56	0.28	0.51	0.20	0.069	0.27	< 0.001	0.76
F: B	1.85	1.35	1.35	1.27	0.115	0.005	0.004	0.064
Others (< 0.5%)	1.69	0.69	0.90	0.46	0.342	0.14	0.046	0.42

OIL, canola oil; 3-NOP, 3-nitrooxypropanol; F: B, Firmicutes: Bacteroidetes
^a,b,cValues within a row with different letters differ (P ≤ 0.05)

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