Application of 3-nitrooxypropanol and canola oil to mitigate enteric methane emissions of beef cattle results in distinctly different effects on the rumen microbial community

Table 4 Impact of feeding diets supplemented with (+) and without (−) 3-NOP and OIL on the protozoal populations (log10 cell/mL) in the rumen fluid of beef at 0, 6 and 12 h after feeding

Item	−3-NOP		+3-NOP		SEM	P value
Item	−OIL	+OIL	−OIL	+OIL	SEM	3-NOP	OIL	3-NOP × OIL
0 h
Isotricha spp.	0.21	ND	0.26	ND	0.200	0.87	0.15	0.87
Dasytricha spp.	0.49	ND	0.54	ND	0.392	0.92	0.016	0.92
Entodiniomorphs
Entodinium spp.	5.60	3.81	5.56	4.28	0.290	0.34	< 0.001	0.27
Ostracodinium spp.	0.47	ND	0.75	ND	0.384	0.50	0.005	0.50
Metadinium spp.	0.95	ND	0.92	0.21	0.562	0.67	< 0.001	0.80
Osphyoscolex spp.	0.68	ND	0.52	ND	0.387	0.69	0.005	0.69
Total	5.61	3.81	5.58	4.28	0.287	0.34	< 0.001	0.27
6 h
Isotricha spp.	0.23	ND	0.24	ND	0.197	0.97	0.15	0.97
Dasytricha spp.	0.49	ND	0.48	ND	0.368	0.98	0.016	0.98
Entodiniomorphs
Entodinium spp.	5.51	4.00	5.49	4.42	0.218	0.21	< 0.001	0.17
Ostracodinium spp.	0.43	ND	0.68	ND	0.349	0.52	0.005	0.52
Metadinium spp.	0.21	ND	0.23	ND	0.185	0.95	0.15	0.95
Osphyoscolex spp.	0.89	ND	0.47	ND	0.403	0.28	0.001	0.28
Total	5.52	4.00	5.50	4.42	0.218	0.21	< 0.001	0.17
12 h
Isotricha spp.	ND	ND	0.23	ND	0.113	0.32	0.32	0.32
Dasytricha spp.	0.24	ND	0.46	ND	0.199	0.59	0.082	0.59
Entodiniomorphs
Entodinium spp.	5.49	3.83	5.47	4.21	0.256	0.48	< 0.001	0.43
Ostracodinium spp.	0.48	ND	0.45	ND	0.225	0.95	0.043	0.95
Metadinium spp.	0.62	ND	0.44	ND	0.225	0.70	0.022	0.70
Osphyoscolex spp.	0.45	ND	0.49	ND	0.228	0.92	0.043	0.92
Total	5.50	3.83	5.48	4.21	0.255	0.48	< 0.001	0.44

ISSN: 2524-4671