Table 1 Composition and concentration of preservation solutions after adding rumen sample
From: Low-cost sample preservation methods for high-throughput processing of rumen microbiomes
Solution | Function | Reagent | Final conc.1 |
|---|---|---|---|
TNx2 | Salt | NaCl | 400Â mM |
Buffering agent | Tris | 10Â mM | |
Surfactant | SDS | 0.6% | |
Chelating agent | EDTA | 100Â mM | |
Bacteriostat | ProClin 300 | 15Â ppm | |
GHx2 | Salt | GuHCl | 1Â M |
Buffering agent | Tris | 100Â mM | |
Surfactant | Tween20 | 3.0% | |
Surfactant | Triton X100 | 0.5% | |
Chelating agent | EDTA | 20Â mM | |
Bacteriostat | ProClin 300 | 15Â ppm | |
EtOH | Preservative | 100% ethanol | 66% |