Table 3 Average DNA yield and quality of sheep rumen samples using four sample preservation methods

DNA yield

Spectrophotometry¹

988 (11)^a

554 (11)^c

857 (11)^b

419 (11)^d

DNA quality

A260/A230

2.26 (0.01)^a

2.22 (0.01)^b

2.25 (0.01)^a

1.96 (0.01)^c

A260/A280

1.91 (0.01)^a

1.87 (0.01)^b

1.87 (0.01)^b

1.88 (0.01)^b

Restriction Enzyme-Reduced Representation Sequencing

Number of failed samples²

0

2

3

13

Number of reads (thousands)³

1014 (12)^a

1004 (11)^a

878 (11)^b

511 (11)^c

Percent of reads assigned^3,4

25 (0.2)^c

27 (0.2)^b

28 (0.2)^a

24 (0.2)^d