Fig. 4

PLS-PM predictions link environmental and maternal microbiota to egg immune function. PLS-PM structural model representations. (A, B) depict predicted path coefficients that were extracted from the global model for experimental microbial environments with A High diversity and B Low diversity. C The experimental treatment effect on path coefficients was assessed with a bootstrap procedure and a t-test, where effects with FDR q < 0.1 were considered significant. A, B Dark grey ellipses depict (uni- or multivariate) latent variables and light grey rectangles represent manifest variables of either reflective or formative indicators of the latent variables. Colored arrows represent the path predictions (blue = positive; red = negative), line weight is proportional to the effect size (arrow labels); asterisks denote the probability that path coefficient is not zero: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001