The microbial environment modulates non-genetic maternal effects on egg immunity

Table 1 Analysis of variance of egg immune function indices

Response	Fixed	df^a	F	P
Albumen lysozyme log-scale (mg ml⁻¹)	Experimental treatment	1, 33	0.01	0.908
	Clutch number	1, 116	4.90	0.029
	Egg sequence	1, 128	0.14	0.709
	pH	1, 122	0.62	0.432

	Fixed	df^a	F	P
Albumen ovotransferrin (mg ml⁻¹)	Experimental treatment	1, 114	2.93	0.090
	Clutch number	1, 114	1.08	0.301
	Egg sequence	1, 114	21.64	< 0.001
	pH	1, 122	0.24	0.627

	Fixed	df^a	F	P
Yolk IgY concentration (absorbance)	Experimental treatment	1, 1.88	0.98	0.433
	Clutch number	1, 118	14.45	< 0.001
	Egg sequence	1, 119	2.62	0.108

	df^a	F	P
Experimental treatment	1, 103	1.36	0.196
Clutch number	1, 103	1.37	0.195
Egg sequence	1, 103	5.02	< 0.001

Bold values denote significant effects (alpha = 0.05)
^aDenominator degrees of freedom based on Satterthwaite approximation
^bDistance-based Redundancy Analysis based on a Bray–Curtis dissimilarity matrix of three immune indices
^cMarginal effects estimated with permutations stratified by female identity

ISSN: 2524-4671