Comparing western (Megascops kennicottii) and whiskered (M. trichopsis) screech-owl microbiomes in southern Arizona using a novel 16S rRNA sequencing method

Bartlow, Andrew W.; Moser, S. Kane; Ellis, Jeremy E.; Hathcock, Charles D.; Fair, Jeanne M.

doi:10.1186/s42523-022-00196-7

Animal Microbiome

Table 1 Metadata for each cloacal and fecal sample sequenced

From: Comparing western (Megascops kennicottii) and whiskered (M. trichopsis) screech-owl microbiomes in southern Arizona using a novel 16S rRNA sequencing method

Year sampled	Sample ID	Bird ID	Species	Sample type	Age	Sex	Box #	# of reads sequenced	Species with most reads (# reads)
2019	1954_001	1613–19835	Whiskered	Cloacal	Nestling	F	13	162,133	Blautia hydrogenotrophica (24,746)
2019	1954_036	1613–19835	Whiskered	Fecal	Nestling	F	13	88,290	Faecalimonas umbilicata (10,193)
2019	1954_003	1613–19834	Whiskered	Cloacal	Nestling	F	13	84,227	Blautia hydrogenotrophica (23,778)
2019	1954_004	1613–19834	Whiskered	Fecal	Nestling	F	13	110,217	Blautia hydrogenotrophica (24,023)
2019	1954_005	1613–19833	Whiskered	Cloacal	Nestling	F	13	108,648	Blautia hydrogenotrophica (22,192)
2019	1954_006	1613–19833	Whiskered	Fecal	Nestling	F	13	147,266	Blautia hydrogenotrophica (19,295)
2019	1954_007	1613–19832	Whiskered	Cloacal	Nestling	F	13	199,876	Blautia hydrogenotrophica (34,544)
2019	1954_008	1613–19832	Whiskered	Fecal	Nestling	F	13	139,362	Faecalimonas umbilicata (20,585)
2019	1954_009	844–74026	Western	Cloacal	Nestling	M	2	126,281	Bifidobacterium cuniculi (19,539)
2019	1954_010	844–74026	Western	Fecal	Nestling	M	2	25,896	Bifidobacterium gallicum (10,319)
2019	1954_011	844–74027	Western	Cloacal	Nestling	F	2	58,921	Collinsella intestinalis (8676)
2019	1954_012	844–74027	Western	Fecal	Nestling	F	2	207,890	Drancourtella massiliensis (16,196)
2019	1954_047	844–21695	Western	Cloacal	Nestling	U	2	15,651	Absiella dolichum (2120)
2019	1954_014	844–21695	Western	Fecal	Nestling	U	2	114,472	Anaeromassilibacillus senegalensis (18,815)
2019	1954_015	874–00013	Whiskered	Cloacal	Adult	F	13	80,968	Collinsella intestinalis (8314)
2019	1954_016	874–00013	Whiskered	Fecal	Adult	F	13	33,431	Collinsella intestinalis (4596)
2019	1954_017	1084–18726	Western	Cloacal	Adult	F	2	200,944	Diplorickettsia massiliensis (79,880)
2019	1954_018	1084–18726	Western	Fecal	Adult	F	2	83,713	Diplorickettsia massiliensis (32,425)
2020	1954_019	874–00014	Whiskered	Fecal	Adult	F	1	98,689	Collinsella intestinalis (21,750)
2020	1954_054	874–00014	Whiskered	Cloacal	Adult	F	1	3399	Collinsella intestinalis (313)
2020	1954_021	1613–19836	Whiskered	Fecal	Adult	F	6	9024	Enorma timonensis (737)
2020	1954_022	1613–19836	Whiskered	Cloacal	Adult	F	6	92,626	Collinsella intestinalis (21,044)
2020	1954_023	1084–18727	Western	Fecal	Nestling	F	12	91,118	Blautia hydrogenotrophica (17,551)
2020	1954_024	1084–18727	Western	Cloacal	Nestling	F	12	48,126	Blautia hydrogenotrophica (8189)
2020	1954_025	1084–18729	Western	Fecal	Nestling	M	12	197,382	Bacteroides fluxus (115,116)
2020	1954_026	1084–18279	Western	Cloacal	Nestling	M	12	155,414	Blautia hydrogenotrophica (23,423)
2020	1954_027	1084–18728	Western	Cloacal	Nestling	F	12	168,507	Drancourtella massiliensis (34,997)
2020	1954_028	1084–18730	Western	Cloacal	Nestling	F	12	62,440	Corynebacterium falsenii (8412)
2020	1954_029	874–00015	Western	Cloacal	Adult	M	12	77,508	Lactobacillus pantheris (20,992)

Nestlings from the same box are siblings, while adults from the same box are parents. Also listed is the number of reads sequenced after filtering for each fecal and cloacal sample and the bacterial species with the most reads identified

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ISSN: 2524-4671

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