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Fig. 4 | Animal Microbiome

Fig. 4

From: Molecular characterization of gut microbiome in weaning pigs supplemented with multi-strain probiotics using metagenomic, culturomic, and metabolomic approaches

Fig. 4

Difference concentration and variation of SCFAs and metabolites compared with MSP and control piglets. Metabolomic analysis of piglet feces at 6 weeks. A SCFA profiling using HPLC–MS/MS. B The results of the volcano plot analysis showed that 7 peak pairs (FC > 1, p ≤ 0.05) were increased, whereas 3 peak pairs (FC < 1, p ≤ 0.05) were reduced. All metabolites that were positively and putatively identified with high confidence were matched using the NIST library. C Principal component analysis (PCA) score plots of metabolites in the feces of piglets between the MSP and control groups. D Selected metabolite and bacterial boxplots showed differences between the MSP and control groups. Mean (SD) values of each group are provided by Student’s t test. Median (IQR) values are provided by the Mann–Whitney U test

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