Fig. 5

Dietary Signature Identification Through GIT Metabarcoding. Relative abundance of dietary components at the phylum level determined through metabarcoding of shotgun metagenomics using the tufA, rbcL, and COI genes (A). Principal coordinate analysis presenting the Bray–Curtis Dissimilarity analysis of the dietary components of seven fish species. Each species’ diet profile grouped separately (PERMANOVA, p = 0.006) (B). Relative abundance of dietary components at the phylum level averaged across samples with error bars representing standard error of the mean (C). Normalized abundance of Metazoan dietary signatures determined by using the COI reads from shotgun metagenomic sequencing of the fish GIT contents (D). Bars represent the average across samples within species and error bars represent standard error of the mean (D). (All data here are from the 69 samples collected in 2021 and do not include previous collections)