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Table 3 Results of global and pairwise test statistics comparing differences in composition of bacterial communities associated with visibly healthy and insulted skin around the gills and visibly healthy skin on the back of black-tip reef sharks (Carcharhinus melanopterus) collected in the Amirante Islands (Seychelles). a) PERMANOVA results under unrestricted permutation to assess statistical differences of location (gills vs. back) of skin bacterial communities. b) Global PERMANOVA results with permutation of residuals under a reduced model to assess statistical differences of sampling site (‘site’), health status (‘health’), and sex of shark (‘sex’) on bacterial community composition on skin around the gills. c) Global PERMANOVA resutls with permutation of residuals under a reduced model to assess statistical differences of sampling site (‘site’), health status (‘health’), and sex of shark (‘sex’) on bacterial community composition on skin on the back. d) Summary of ANOSIM pairwise tests for ‘site’. Global R = 0.551, significance level p < 0.0001

From: Similar bacterial communities on healthy and injured skin of black tip reef sharks

PERMANOVA table of results
a) Pairwise PERMANOVA (gills vs. back)
Terms added sequentially (first to last)
Df Su SS MS F.Model R2 Pr(>F)
 Skin 1 0.2019 0.20193 0.20193 0.8359 0.0963 0.5657
 Residuals 86   20.7746 0.24157 0 99,037  
 Total 87   20.9765   1 0  
b) global PERMANOVA for gill samples
Terms added sequentially (first to last)
  Df SS MS F.Model R2 Pr(>F)
 Site 4 3.787 0.94675 5.5281 0.3651 0.0001
 Health 1 0.2015 0.20145 1.1763 0.01942 0.2666
 Sex 1 0.1692 0.16915 0.9877 0.01631 0.4169
 Site:Health 3 0.4111 0.13705 0.8002 0.03964 0.7672
 Site:Sex 4 0.7226 0.18064 1.0548 0.06966 0.3818
 Health:Sex 1 0.1146 0.11461 0.6692 0.01105 0.7496
 Residuals 29 4.9666 0.17126   0.47882  
 Total 43 10.3725    1  
c) global PERMANOVA for back samples
Terms added sequentially (first to last)
  Df SS MS F.Model R2 Pr(>F)
 Site 4 3.5699 0.89246 4.9904 0.34319 0.0001
 Health 1 0.195 0.19504 1.0906 0.01875 0.3035
 Sex 1 0.2623 0.26227 1.4665 0.02521 0.1232
 Site:Health 3 0.4142 0.13808 0.7721 0.03982 0.8355
 Site:Sex 4 0.6674 0.16684 0.9329 0.06416 0.5962
 Health:Sex 1 0.1071 0.10709 0.5988 0.01029 0.853
 Residuals 29 5.1862 0.17884   0.49858  
 Total 43 10.4021     
d) ANOSIM for gill/back samples
Pairwise Tests
Groups R Stats Sig. Level   Act. Perm.  
 StJos_WRes, D’Arros_North 0.8415/0.6811 0.001/0.001   9999  
 StJos_WRes, StJos_ERes 0.3809/0.4359 0.002/0.001   9999  
 StJos_WRes, StJos_Fouq 0.3692/0.2233 0.002/0.024   9999  
 StJos_WRes, StJos_Ben 0.4525/0.1103 0.001/0.241   9999  
 D’Arros_North, StJos_ERes 0.8476/0.8237 0.001/0.001   9999  
 D’Arros_North, StJos_Fouq 0.8923/0.7936 0.001/0.001   9999  
 D’Arros_North, StJos_Ben 0.9164/0.7095 0.003/0.007   9999  
 StJos_North, StJos_Fouq 0.01162/0.01265 0.364/0.371   9999  
 StJos_ERes, StJos_Ben 0.1076/0.3354 0.270/0.086   9999  
 StJos_Fouq, StJos_Ben 0.0119/0.1746 0.436/0.184   9999