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Table 3 Results of global and pairwise test statistics comparing differences in composition of bacterial communities associated with visibly healthy and insulted skin around the gills and visibly healthy skin on the back of black-tip reef sharks (Carcharhinus melanopterus) collected in the Amirante Islands (Seychelles). a) PERMANOVA results under unrestricted permutation to assess statistical differences of location (gills vs. back) of skin bacterial communities. b) Global PERMANOVA results with permutation of residuals under a reduced model to assess statistical differences of sampling site (‘site’), health status (‘health’), and sex of shark (‘sex’) on bacterial community composition on skin around the gills. c) Global PERMANOVA resutls with permutation of residuals under a reduced model to assess statistical differences of sampling site (‘site’), health status (‘health’), and sex of shark (‘sex’) on bacterial community composition on skin on the back. d) Summary of ANOSIM pairwise tests for ‘site’. Global R = 0.551, significance level p < 0.0001

From: Similar bacterial communities on healthy and injured skin of black tip reef sharks

PERMANOVA table of results

a) Pairwise PERMANOVA (gills vs. back)

Terms added sequentially (first to last)

Df

Su

SS

MS

F.Model

R2

Pr(>F)

 Skin

1

0.2019

0.20193

0.20193

0.8359

0.0963

0.5657

 Residuals

86

 

20.7746

0.24157

0

99,037

 

 Total

87

 

20.9765

 

1

0

 

b) global PERMANOVA for gill samples

Terms added sequentially (first to last)

 

Df

SS

MS

F.Model

R2

Pr(>F)

 Site

4

3.787

0.94675

5.5281

0.3651

0.0001

 Health

1

0.2015

0.20145

1.1763

0.01942

0.2666

 Sex

1

0.1692

0.16915

0.9877

0.01631

0.4169

 Site:Health

3

0.4111

0.13705

0.8002

0.03964

0.7672

 Site:Sex

4

0.7226

0.18064

1.0548

0.06966

0.3818

 Health:Sex

1

0.1146

0.11461

0.6692

0.01105

0.7496

 Residuals

29

4.9666

0.17126

 

0.47882

 

 Total

43

10.3725

  

1

 

c) global PERMANOVA for back samples

Terms added sequentially (first to last)

 

Df

SS

MS

F.Model

R2

Pr(>F)

 Site

4

3.5699

0.89246

4.9904

0.34319

0.0001

 Health

1

0.195

0.19504

1.0906

0.01875

0.3035

 Sex

1

0.2623

0.26227

1.4665

0.02521

0.1232

 Site:Health

3

0.4142

0.13808

0.7721

0.03982

0.8355

 Site:Sex

4

0.6674

0.16684

0.9329

0.06416

0.5962

 Health:Sex

1

0.1071

0.10709

0.5988

0.01029

0.853

 Residuals

29

5.1862

0.17884

 

0.49858

 

 Total

43

10.4021

    

d) ANOSIM for gill/back samples

Pairwise Tests

Groups

R Stats

Sig. Level

 

Act. Perm.

 

 StJos_WRes, D’Arros_North

0.8415/0.6811

0.001/0.001

 

9999

 

 StJos_WRes, StJos_ERes

0.3809/0.4359

0.002/0.001

 

9999

 

 StJos_WRes, StJos_Fouq

0.3692/0.2233

0.002/0.024

 

9999

 

 StJos_WRes, StJos_Ben

0.4525/0.1103

0.001/0.241

 

9999

 

 D’Arros_North, StJos_ERes

0.8476/0.8237

0.001/0.001

 

9999

 

 D’Arros_North, StJos_Fouq

0.8923/0.7936

0.001/0.001

 

9999

 

 D’Arros_North, StJos_Ben

0.9164/0.7095

0.003/0.007

 

9999

 

 StJos_North, StJos_Fouq

0.01162/0.01265

0.364/0.371

 

9999

 

 StJos_ERes, StJos_Ben

0.1076/0.3354

0.270/0.086

 

9999

 

 StJos_Fouq, StJos_Ben

0.0119/0.1746

0.436/0.184

 

9999

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