Fig. 6

Metabolites composition and the differential analysis in both stool and ascidian tissue samples. a The PCA analysis of all the samples according to the abundance of metabolites. The green dots indicate stool samples before starvation (Day 0 stool); the green rectangles indicate stool samples with starvation for 2, 4, and 6 days (Day 246 stool); the blue dots indicate the tissue samples from H. roretzi before starvation (Day 0 tissue); the blue rectangles indicate tissue samples with starvation for 2, 4, and 6 days (Day 246 tissue). b The number of the differentially expressed metabolites between different groups. The red columns indicate the number of upregulated metabolites and the blue columns indicate the number of downregulated metabolites. c The abundance heatmap of the metabolites identified from both stool and tissue samples. The red color indicates the relatively high expression while the blue color indicates the relatively low expression. d The bubble plots of significantly different metabolites enriched pathways between different groups. The x-axis indicates the impact and the y-axis indicates the -ln P-value