Table 2 BRD pathobiont primers and probes sequences for PCR and qPCR reactions
From: Identification of bovine respiratory disease through the nasal microbiome
Target | Target gene | Primer name | Sequence (5′–3′) | Size (bp) | Ref |
|---|---|---|---|---|---|
M. haemolytica | sodA | Mh-SGF | AGCAGCGACTACTCGTGTTGGTTCAG | 26 | [80] |
M. haemolytica | sodA | Mh-SGR | AAGACTAAAATCGGATAGCCTGAAACGCCTG | 31 | [80] |
M. haemolytica | sodA | Mh-BV1P* | TTCAACCGCTAACCAGGACAACCCAC | 26 | [80] |
P. multocida | 16S rRNA | Pm-TMF | CGCAGGCAATGAATTCTCTTC | 21 | [81] |
P. multocida | 16S rRNA | Pm-TMR | GGCGCTCTTCAGCTGTTTTT | 20 | [81] |
P. multocida | 16S rRNA | Pm-TMP* | ACTGCACCAACAAATGCTTGCTGAGTTAGC | 30 | [81] |
H. somni | 16S rRNA | Hs-TMF | AGGAAGGCGATTAGTTTAAGAGATTAATT | 29 | [81] |
H. somni | 16S rRNA | Hs-TMR | TCACACCTCACTTAAGTCACCACCT | 25 | [81] |
H. somni | 16S rRNA | Hs-TMP* | ATTGACGATAATCACAGAAGAAGCACCGGC | 30 | [81] |
M. bovis | oppD | PMB996-F | TCAAGGAACCCCACCAGAT | 19 | [79] |
M. bovis | oppD | PMB1066-R | AGGCAAAGTCATTTCTAGGTGCAA | 24 | [79] |
M. bovis | oppD | Mbovis1016* | TGGCAAACTTACCTATCGGTGACCCT | 26 | [79] |