Table 1 Sample types included in bacterial 16S rRNA gene sequencing
Sample type | Sample type definition | n per genotype* | Total no. of samples |
|---|---|---|---|
Hatched larvae | Surface sterilized newly hatched larvae analyzed as whole | 4 | 8 |
Washing water | Water from washing newly hatched larvae | 4 | 8 |
Control diet | Artificial diet | na | 2 |
Transplant diet | Mix of control diet and frass | 2 | 4 |
Frass before | Frass from larvae fed with control diet before transplantation | 2 | 4 |
Frass after | Frass from larvae fed with transplant diet of the other genotype | WW: 3, yy: 2 | 5 |
Frass after control | Frass from larvae fed with transplant diet of the same genotype | 2 | 4 |
Gut | Gut of adult from larvae fed with transplant diet of the other genotype | WW: 2, yy: 1 | 3 |
Gut control | Gut of adult from larvae fed with transplant diet of the same genotype | WW: 3, yy: 2 | 5 |
Abdominal fluid | Abdominal fluid of adult from larvae fed with transplant diet of the other genotype | WW: 10, yy: 4 | 14 |
Abdominal fluid control | Abdominal fluid of adult from larvae fed with transplant diet of the same genotype | WW: 8, yy: 4 | 12 |
Water control | Water used in artificial diet and sample storage | na | 3 |