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Fig. 3 | Animal Microbiome

Fig. 3

From: Short-term feeding of defatted bovine colostrum mitigates inflammation in the gut via changes in metabolites and microbiota in a chicken animal model

Fig. 3

Decreased protein tyrosine nitration (NT) in epithelial cells of villi in samples of ileum from chickens fed a BC-supplemented diet. Immunofluorescence is presented in Panels A–F and Image analysis depicted in Panels G–I. Epithelial cell (anti-cytokeratin 18 epithelial cell marker) presence is indicated by green fluorescence pixels (A, C). NT, as detected using an anti-nitrotyrosine antibody marker, is presented as red (cell presence, B and D–F, for isolated single channel image analysis quantification) or orange (colocalized in green cytokeratin 18-positive cells, C Representative patterns of image analysis-selected nitrotyrosine pixels (G–I, yellow pixels) were summated and processed to yield the statistical analysis summarized in the lower panel bar graph(J). Values are group means ± SEM for Control (n = 6), NFDM (n = 6), and BC (n = 10)

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Animal Microbiome

ISSN: 2524-4671