Growth performance, caecal microbiome profile, short-chain fatty acids, and litter characteristics in response to placement on reused litter and combined threonine, arginine and glutamine supplementation to juvenile male broiler chickens

Table 6 Primers for PCR and qPCR with PCR conditions

Pathogen	Target	Primer and probe sequences (5′–3)	Tm* (°C)	Amplicon size (bp)	Ref**
Salmonella	ttr-4	F:AGCTCAGACCAAAAGTGACCATC	66	94	[74]
Salmonella	ttr-4	R:CTCACCAGGAGATTACAACATGG	66	94	[74]
C. perfringens	16S rRNA	F:GGGGGTTTCAACACCTCC	63	170	[75]
	16S rRNA	R:GCAAGGGATGTCAAGTGT	63	170	[75]
	CPα	F:GCTAATGTTACTGCCGTTGA	60	324	[76]
	CPα	R:CCTCTGATACATCGTGTAAG	60	324	[76]
	NetB	F:GCTGGTGCTGGAATAAATGC	65	383	[77]
	NetB	R:TCGCCATTGAGTAGTTTCCC	65	383	[77]
E. tenella	ITS1	F:AATTTAGTCCATCGCAACCCTTG	65	279	[78]
E. tenella	ITS1	R:CGAGCGCTCTGCATACGACA	65	279	[78]
E. maxima	ITS1	F:GTGGGACTGTGGTGATGGGG	65	205	[78]
E. maxima	ITS1	R:ACCAGCATGCGCTCACAACCC	65	205	[78]
Total bacteria	16S rRNA	F:ACTCCTACGGGAGGCAGCAGT	60	194	[80]
		R:TATTACCGCGGCTGCTGGC
		Probe:CGCGTGACCCTTATTGCTCCACA

ISSN: 2524-4671