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Fig. 1 | Animal Microbiome

Fig. 1

From: Agaricus subrufescens fermented rye affects the development of intestinal microbiota, local intestinal and innate immunity in suckling-to-nursery pigs

Fig. 1

Experimental set up. Piglets orally received either ROM or tap water (Ctrl group) every other day, starting from day 2 until day 44. An oral vaccine (Salmoporc®) was given on day 21 and day 45 (booster vaccination). A randomly selected subset of animals (eight per group) were euthanised on 3 days (days 27, 44 and 70). Body weight (BW) was monitored for pig growth performance. Faeces and digesta from jejunum, ileum and caecum were collected and processed for 16S rRNA gene sequencing. Ileal and caecal mucosa were sampled for immune gene expression analysis. Blood samples were taken to assess vaccine-specific antibodies (IgM, IgA and IgG) against Salmonella typhimurium (Salmoporc®). Furthermore, blood samples were processed and evaluated for immune cells, and mesenteric lymph node (MLN) samples were re-stimulated with the mitogen LPS

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Animal Microbiome

ISSN: 2524-4671