Table 1 Published studies addressing the taxonomic composition of the bacterial microbiota associated with H. illucens
References | Rearing conditions | Substrates | Life stage | Tissue | Methodology |
|---|---|---|---|---|---|
Jeon et al. 2011 [38] | Not specified | Food waste; calf forage; cooked rice | Larvae | Whole gut | Pyrosequencing |
Zheng et al. 2013 [57] | 27 °C RH: Ambient | Gainesville diet | Eggs, larvae, prepupae, pupae, adults | Whole individual | Pyrosequencing |
Zheng et al. 2013 [58] | 27 °C RH: Ambient | Gainesville diet | Eggs and larvae | Whole individual | Pyrosequencing; cultivation followed by partial 16 S rRNA gene sequencing |
Cai et al. 2018 [60] | 27 °C RH: 70% | Chicken manure | Larvae | Whole gut | Illumina HiSeq amplicon sequencing |
Cai et al. 2018 [61] | 27.5 °C RH: 70% | Wheat bran | Larvae | Whole gut | Illumina HiSeq amplicon sequencing; cultivation followed by partial 16 S rRNA gene sequencing |
Bruno et al. 2019 [39] | 27 °C RH: 70% | Mixture of vegetable flours; vegetables; fish meal | Larvae | Midgut (anterior, middle, and posterior regions) | Illumina MiSeq amplicon sequencing |
Jiang et al. 2019 [30] | Not specified | Raw food waste | Larvae | Whole gut | Illumina HiSeq amplicon sequencing |
Zhan et al. 2019 [41] | 25 °C 35–40% | Food waste; poultry manure; dairy manure; swine manure | Larvae | Midgut | Illumina HiSeq amplicon sequencing |
Wynants et al. 2019 [40] | 24.9–29.1 °C RH: 21–71% | Fruit/vegetable waste; supermarket and restaurant waste; poultry blood; poultry manure; chicken feed; wheat bran; wheat flour; yeast concentrate; brewer’s spent grains | Larvae | Whole individual | Illumina MiSeq amplicon sequencing |
Ao et al. 2020 [43] | 27.5 °C RH: 70% | Mixture of wheat flour and bran; swine/chicken manure | Larvae | Whole gut | Illumina MiSeq amplicon sequencing |
Callegari et al. 2020 [80] | 25 °C RH: 60–65% | Mixture of wheat germ, alfalfa and corn flour | Larvae | Whole gut | Cultivation followed by partial 16 S rRNA gene sequencing |
Cifuentes et al. 2020 [62] | 25 °C RH: Not specified | Commercial chicken feed | Larvae and prepupae | Whole gut | Illumina MiSeq amplicon sequencing |
Khamis et al. 2020 [63] | Not specified | Not specified | Larvae | Whole individual | Illumina MiSeq amplicon sequencing |
Klammsteiner et al. 2020 [42] | 27 °C RH: 60% | Chicken feed; freshly cut grass; vegetables | Larvae | Whole gut | Illumina MiSeq amplicon sequencing |
Liu at al. 2020 [64] | 29 °C RH: Not specified | Soya meal | Larvae | Whole gut | Illumina MiSeq amplicon sequencing |
Raimondi et al. 2020 [65] | 20–33 °C RH: 65% | Mixture of vegetable flours | Larvae and prepupae | Whole individual | Illumina MiSeq amplicon sequencing; cultivation followed by identification on specific Agar plates |
Shelomi et al. 2020 [54] | Not specified | Postproduction soy pulp; postconsumer cafeteria waste | Larvae | Whole gut | Illumina HiSeq amplicon sequencing; cultivation followed by partial 16Â S rRNA gene sequencing |
Wu et al. 2020 [74] | 30 °C RH: Not specified | Wheat bran | Larvae | Whole gut | Illumina MiSeq amplicon sequencing |
Galassi et al. 2021 [55] | 25 °C RH: 60% | Hen diet; okara; maize distillers; brewer’s grains | Larvae | Whole gut | Illumina MiSeq amplicon sequencing |
Tegtmeier et al. 2021 [44] | 27 °C RH: 65% | Chicken feed; cottonseed press cake | Larvae | Whole gut | Illumina MiSeq amplicon sequencing |
Tegtmeier et al. 2021 [66] | 27 °C RH: 65% | Chicken feed | Larvae | Whole gut | Illumina MiSeq amplicon sequencing; cultivation followed by partial 16 S rRNA gene sequencing |
Gorrens et al. 2021 [45] | 27 °C RH: 60% | Chicken feed; fiber-rich ingredients | Larvae | Whole individual | Cultivation followed by identification with mass spectrometry |
Greenwood et al. 2021 [46] | 30 °C RH: 64% | Grain-based feed; mixture of brewer’s spent grain and a plant-based sweetener; vegetable waste stream | Larvae | Whole gut | Ion Torrent sequencing |
Klammsteiner et al. 2021 [47] | 27 °C RH: 60% | Chicken feed; food waste; oil wast | Larvae | Whole gut | Illumina MiSeq amplicon sequencing |
Li et al. 2021 [73] | 28 °C RH: 60–70% | Food waste | Larvae | Whole gut | Illumina MiSeq amplicon sequencing; cultivation followed by partial 16 S rRNA gene sequencing |
Liu et al. 2021 [67] | 29 °C RH: 65% | Soya meal; oxytetracycline bacterial residue | Larvae | Whole gut | Illumina MiSeq amplicon sequencing |
Osimani et al. 2021 [48] | 27 °C RH: 65% | Mixtures of coffee by-products and microalgae | Larvae | Whole individual | Illumina MiSeq amplicon sequencing |
Shumo et al. 2021 [49] | 28 °C RH: 65% | Chicken manure; kitchen waste | Larvae | Whole individual | Oxford Nanopore MinION Sequencing; cultivation followed by partial 16 S rRNA gene sequencing |
Tanga et al. 2021 [50] | Ambient conditions | Brewers’ spent grains; kitchen food waste; poultry manure; rabbit manure | Larvae | Whole gut | Illumina MiSeq amplicon sequencing |
Yang et al. 2021 [53] | 27 °C RH: 70% | Gainesville diet; starvation | Larvae | Whole individual | Illumina HiSeq amplicon sequencing |
Zhang et al. 2021 [68] | 30 °C RH: Not specified | Chicken manure | Larvae | Whole gut | Illumina MiSeq amplicon sequencing |
Zhineng et al. 2021 [51] | 25 °C RH: 60–70% | Wheat bran + soybean powder; food waste | Larvae | Whole gut | Metagenomic sequencing (approach not specified) |
Soomro et al. 2021 [56] | 27 °C RH: 70% | Corn flour + bran | Larvae | Whole individual | Illumina HiSeq amplicon sequencing |
Cifuentes et al. 2022 [79] | 25 °C RH: Not specified | Chicken feed | Larvae | Whole gut | Cultivation followed by partial 16 S rRNA gene sequencing |
Gorrens et al. 2022 [52] | 30 °C / ambient temperature RH: 50–60% | Chiken feed; mixture of vegetable coproducts; pig feed; pig feed + wheat bran | Larvae | Whole individual | Illumina MiSeq amplicon sequencing |
Pei at al. 2022 [69] | 30 °C RH: 70% | Wheat bran; food waste + peanut shell powder | Larvae | Whole gut | Illumina MiSeq amplicon sequencing |
Querejeta et al. 2022 [70] | Not specified | Gainesville diet | Eggs, larvae, prepupae, pupae, adults | Whole gut | Illumina MiSeq amplicon sequencing |
Zhang et al. 2022 [72] | 27 °C RH: 60–70% | Pig manure | Larvae | Whole gut | Illumina MiSeq amplicon sequencing; cultivation followed by partial 16 S rRNA gene sequencing |
Yu et al. 2023 [71] | 28 °C RH: Not specified | Artificial diet for Drosophila melanogaster | Larvae | Whole gut | Illumina MiSeq amplicon sequencing |