Table 2 The primer names, target organism, amplified gene, sequences, annealing temperatures, and references are listed for each COI and endosymbiotic primer pair that was used in our research

BCW

mtDNA COI

RF_450F

5`-ACCTGATATGGCTTTTCCCCG-3`

58 ° C

[79]

RF_1123R

5`-ACCAAGAATTCCAAAGGTTTCTTT-3`

all Eubacteria

16 S rRNA

Bact1369F

5`- CGGTGAATACGTTCYCGG-3`

50 °C

[92].

Prok1492R

5`- GGWTACCTTGTTACGACTT-3`

Wolbachia

Wsp

wsp_F1

5`-GTCCAATARSTGATGARGAAAC- 3`

56 °C

[10].

wsp_R1

5`-CYGCACCAAYAGYRCTRTAAA − 3`

Rickettsia

gltA

RicF141

5’-TCGGTTCTCTTTCGGCATTTTA-3’

56 °C

[35].

RicR548

5’ -GCATATTTATCACCGCTTCATT-3’

Spiroplasma

23 S rDNA

SP-ITS-JO4

5’-GCCAGAAGTCAGTGTCCTA ACCG-3’

56 °C

[57].

SP-ITS-N55

5’-ATTCCAAGCCATCC ACCATACG-3’