Table 1 Significant pathway relative abundances in the control group (n = 30) versus the Cryptosporidium-positive group (n = 30).
MetaCyc pathway | Biological function | Coefficient value* | Association with C. parvum | Taxa contributing to pathway | p-value | q-value (adjusted p-value) |
|---|---|---|---|---|---|---|
TEICHOICACID-PWY: teichoic acid (poly-glycerol) biosynthesis | Interacts with isoprenoids and forms Gram-positive bacterial cell wall | 1.81E−04 | Positive | Escherichia coli, Staphylococcus condimenti, unclassified | 0.002293 | 0.020765 |
PWY-5920: superpathway of haem biosynthesis from glycine | Cytochromes use haem as a cofactor | 3.20E−04 | Positive | Escherichia coli, unclassified | 0.007606 | 0.057446 |
PWY-7392: taxadiene biosynthesis (engineered) | Intermediate of isoprenoid biosynthesis converts to taxol precursor | 1.59E−04 | Positive | Unclassified | 0.009125 | 0.066171 |
PWY-7560: methylerythritol phosphate pathway II | Isoprenoid precursor biosynthesis | 4.15E−04 | Positive | Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, unclassified | 0.014846 | 0.102318 |
PWY-5695: urate biosynthesis/inosine 5-monophosphate degradation | Purine nucleotide degradation | − 6.14E−04 | Negative | Alistipes, Allisonella, Anaerostipes, Anaerotignum, Bacteroides, Bibersteinia, Bilophila, Ruminococcus, Catenibacterium, Citrobacter, Clostridioides, Clostridium, Desulfovibrio, Enterobacter, Enterococcus, Clostridium, Escherichia, Flavonifractor, Fusicatenibacter, Fusobacterium, Gallibacterium, Hafnia, Intestinibacter, Klebsiella, Kluyvera, Kocuria, Lactococcus, Mannheimia, Megamonas, Megasphaera, Morganella, Parabacteroides, Pasteurella, Prevotella, Proteus, Providencia, Pseudoflavonifractor, Raoultella, Staphylococcus, Streptococcus, Terrisporobacter, Vagococcus, Veillonella, and unclassified spp. (Species are grouped by genera here for brevity) | 0.016182 | 0.110565 |
PWY-5121: superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP) | Isoprenoid precursor biosynthesis | 2.39E−04 | Positive | Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, unclassified | 0.017217 | 0.115303 |
PWY-6859: all-trans-farnesol biosynthesis | Isoprenoid precursor biosynthesis | 1.75E−04 | Positive | Citrobacter portucalensis, Citrobacter youngae, Enterobacter cloacae complex, Escherichia coli, Escherichia fergusonii, Klebsiella oxytoca, Klebsiella pneumonia, Morganella morganii, Proteus mirabilis, Proteus vulgaris, Providencia stuartii, unclassified | 0.01747 | 0.116663 |
NONMEVIPP-PWY: methylerythritol phosphate pathway I | Isoprenoid precursor biosynthesis | 4.72E−04 | Positive | Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, unclassified | 0.019814 | 0.128331 |
PWY66-409: superpathway of purine nucleotide salvage | Purine nucleotide salvage | 4.86E−04 | Positive | Clostridium butyricum, Clostridium perfringens, Clostridium sp 7 2 43FAA, Enterobacter cloacae complex, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella oxytoca, Klebsiella pneumonia, Proteus mirabilis, Providencia stuartii, Streptococcus equinus, Streptococcus gallolyticus, Streptococcus pasteurianus, unclassified | 0.023986 | 0.15325 |
PWY-6270: isoprene biosynthesis I | Isoprenoid precursor biosynthesis | 2.73E−04 | Positive | Escherichia coli, Klebsiella pneumonia, Proteus mirabilis, unclassified | 0.034367 | 0.206205 |
PWY-6383: mono-trans, poly-cis decaprenyl phosphate biosynthesis | Isoprenoid precursor biosynthesis | 1.35E−04 | Positive | Unclassified | 0.035707 | 0.212223 |
PWY-5910: superpathway of geranylgeranyl diphosphate biosynthesis I (via mevalonate) | Isoprenoid precursor biosynthesis | 3.49E−05 | Positive | Unclassified | 0.039785 | 0.229955 |