Fig. 4

Microbial community composition of Aurelia aurita polyps under environmental challenge. Microbial communities were analyzed by sequencing the V1-V2 region of the bacterial 16S rRNA gene. OTU abundances were summarized at the genus level and normalized by the total number of reads per sample. (A) Bar plots visualizing the dominant genera (reaching at least 1% of relative abundance) after 14 days of maintenance under control and at the indicated conditions. All data are based on the means of 6 biological replicates. (B) Differences in relative abundances at day 14 compared to control conditions for all dominant genera. (C) Boxplot of alpha-diversity Shannon indices. (D) Principal component analysis (PCA) for genus-level microbiota of the polyps (replicates of the same treatment are grouped with polygons). Different colors and shapes denote treatments and ellipses representing 95% confidence interval for the centroids of each data cluster