Fig. 5

Fecal resistome composition of dairy cows during the 9-week study. A) The normalized abundance score and B) Shannon Index comparing antibiotic-treated (orange) and control (grey) animals is shown. Each boxplot includes the median, lower, and upper quartiles with the whiskers representing extreme values in the distribution. C) The resistome composition at the drug class level shows the average proportion; “n” indicates the number of genes assigned to a given class. D) PCoAs of the Bray-Curtis dissimilarity clustered by sampling point (ellipses contain at least 90% of the samples). P-values were calculated using a paired Wilcoxon signed rank test to compare treatment groups within a sampling point