Table 2 Study design and actions performed to followed piglets born to treated or non-treated sows
Production phase | Day (D) | Action performed | Analysis | Non-treated sow | Treated-sow | |||
|---|---|---|---|---|---|---|---|---|
Control (N = 10) | TP (N = 9) | IP (N = 12) | TS (N = 11) | TS-IP (N = 12) | ||||
Lactation | D0 | Piglet treatment | Â | 0 | 115 | 0 | 0 | 0 |
Piglet inoculation | 0 | 0 | 175 | 0 | 154 | |||
Weight | 118 | 115 | 175 | 129 | 154 | |||
Nasal sampling | M. hyorhinis, G. parasuis and S. suis PCRsa | 0 | 10 | 0 | 10 | 0 | ||
Microbiota 16S sequencing | 0 | 5 | 0 | 5 | 0 | |||
D7 | Nasal sampling | G. parasuis and S. suis cultureb | 10 | 9 | 12 | 11 | 12 | |
M. hyorhinis, G. parasuis and S. suis PCRsc | 50 | 45 | 60 | 55 | 60 | |||
Microbiota 16S sequencing | 5 | 5 | 5 | 5 | 5 | |||
D15 | Nasal sampling | G. parasuis and S. suis cultureb | 10 | 9 | 12 | 11 | 12 | |
M. hyorhinis, G. parasuis and S. suis PCRsc | 50 | 45 | 60 | 55 | 60 | |||
Nursery | D21 | Weight | Â | 101 | 85 | 118 | 89 | 97 |
Nasal sampling | G. parasuis and S. suis cultureb | 10 | 9 | 12 | 11 | 12 | ||
M. hyorhinis, G. parasuis and S. suis PCRsc | 50 | 45 | 60 | 55 | 60 | |||
Microbiota 16S sequencing | 5 | 5 | 5 | 5 | 5 | |||
WGS metagenomics | 5 | 5 | 5 | 5 | 5 | |||
D49 | Nasal sampling | G. parasuis and S. suis cultureb | 10 | 9 | 12 | 11 | 12 | |
M. hyorhinis, G. parasuis and S. suis PCRsc | 25 | 20 | 25 | 25 | 25 | |||
Microbiota 16S sequencing | 5 | 5 | 5 | 5 | 5 | |||
WGS metagenomics | 5 | 5 | 5 | 5 | 5 | |||