Fig. 8
Meta-pangenomic analysis of Sulfitobacter sp. reveals its coverage in microbiota before and post gut development. a Pangenomics of Sulfitobacter sp. indicated the unique and core genes in BL28, and the higher total genome coverage in the post gut development. The inner radial dendrogram shows the gene clusters in the pangenome, clustered by presence/absence across genomes. The four genomes of Sulfitobacter strains are plotted on the innermost four layers, spaced to reflect discernable groups based on genomic composition. The genome pointed in orange is BL28 isolated from sea cucumber microbiome. Gene clusters within a given genome are filled in with black or dark orange; gene clusters do not present remain unfilled or light orange. Core gene across the Sulfitobacter strains were pointed in red and unique gene detected in BL28 were pointed in orange. Above the genome content summaries, each genome’s median coverage across larval metagenomes with different gut development status is shown in the colored bar graph. ANI value of genome is shown in the heatmap above, where each row represents a different sample, and cell color intensity reflects the ANI value. The colored two layers show the proportion of genes within each gene cluster determined to be environmental accessory or core genes: EAGs (green) and ECGs (blue) with 23 metagenomes. b Mean coverage of each gene in the pangenome of Sulfitobacter sp. within two developments indicated higher coverage in metagenomes of post-gut development environment. Outer layers show the four genomes of Sulfitobacter spp.and the coverage in environmental metagenomes. PGD, post gut development; BGD, before gut development