Characterization of the hoof bacterial communities in feedlot cattle affected with digital dermatitis, foot rot or both using a surface swab technique

Table 2 ALDEx2 output of differential abundant bacterial taxa in specific lesion type pairwise comparisons

Taxon	Lesion type	Control CLR	Lesion CLR	Effect size	Adj P value
Peptostreptococcaceae	DD	0.85	7.1	1.44	< 0.01
Mycoplasma	DD	− 1.53	6.94	1.24	< 0.01
Amnipila	DD	− 3.18	7.09	0.98	< 0.01
Acholeplasma	DD	− 0.64	5.7	0.94	< 0.01
Porphyromonas	DD	6.23	9.36	0.9	< 0.01
Murdochiella	DD	− 3.06	4.34	0.89	0.014
Fusobacterium	DD	− 2.84	4.59	0.87	< 0.01
Treponema	DD	4.93	9.82	0.87	0.032
Roseburia	DD	− 2.21	6.84	0.85	0.016
Campylobacter	DD	− 1.48	4.65	0.81	0.017
Filifactor	DD	− 3.59	0.98	0.8	0.013
Fusobacterium	DD + FR	− 2.43	8.71	2.12	< 0.01
Porphyromonas	DD + FR	3.85	9.53	1.32	< 0.01
Succiniclasticum	DD + FR	− 2.49	6.55	1.2	< 0.01
Peptostreptococcus	DD + FR	− 2.28	6.24	1.13	< 0.01
Peptoniphilus	DD + FR	2.95	7.43	1.07	< 0.01
Peptococcus	DD + FR	− 2.28	3.78	1.07	< 0.01
Helcococcus	DD + FR	1.26	6.07	0.99	< 0.01
Campylobacter	DD + FR	− 2.47	4.35	0.93	< 0.01
S5-A14a	DD + FR	− 3.2	3.48	0.85	0.011
Mycoplasma	DD + FR	− 3.14	5.37	0.84	< 0.01
[Eubacterium]_yurii_group	DD + FR	− 2.53	4.07	0.8	< 0.01
Lachnospiraceae_NK3A20_group	DD + FR	5.22	− 1.21	− 1.18	< 0.01
Fusobacterium	FR	− 1.45	9.45	1.05	< 0.01
Porphyromonas	FR	4.47	9.93	1.02	< 0.01
Peptostreptococcus	FR	− 1.96	7.04	0.9	< 0.01

CLR—median value of a genus’s transformed abundance data in the lesion or control skin group of samples; a higher CLR indicates a higher abundance
Adj P value—Benjamini–Hochberg adjusted P value controlled for false discovery rate
ALDEx2 transforms the compositional abundance data into centered log ratios (CLR) for taxa in the lesion and control skin group. It then compares them using a two-sample Welch’s t-test to determine significance. Only significant taxa with adj P value < 0.05 and the effect size > 0.8 are shown

ISSN: 2524-4671