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Table 10 Cycling methods for RT-PCR, including run stage with associated temperature and duration

From: Alterations to the bovine bacterial ocular surface microbiome in the context of infectious bovine keratoconjunctivitis

Run Stage

Temperature

Time

Hold

50℃

2 min

95℃

10 min

PCR (40 cycles)

95℃

15 s

60℃

10 min

Continuous melt curve

95℃

15 s

60℃

15 s

Dissociation

95℃

15 s

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Animal Microbiome

ISSN: 2524-4671