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Table 5 Relative DNA quantities obtained by PCR and evaluated by Mann-Whitney U tests. Asterisk (*) indicates significant difference with respect to disease status

From: Alterations to the bovine bacterial ocular surface microbiome in the context of infectious bovine keratoconjunctivitis

 

Median (Min-Max) Log DNA (ag = 10− 18 g) per 10 ng isolated total DNA

Target Primer

Disease status

Normal (n = 369)

IBK (n = 227)

Bov. GAPDH

7.18 (4.99–8.41)

7.23 (4.85–7.83)

Moraxella bovis

3.73 (0.00-6.63)*

5.09 (0.00-8.34)*

Moraxella bovoculi

3.79 (0.00-7.10)*

5.14(0.00-7.63)*

Mycoplasma

7.20 (3.72–8.85)

7.21(3.74–9.16)

Staphylococcus

4.60 (0.00-9.19)*

3.83 (0.00-9.68)*

Pasteurellaceae

4.76 (0.00-7.85)*

5.37 (0.00-8.42)*

Prevotellaceae

6.01 (4.48–8.41)

6.12(4.64–8.10)

Weeksellaceae

6.14 (0.00–9.00)*

6.38 (0.00-8.04)*

Universal bacteria

8.19 (6.63–10.30)

8.23 (6.85–10.30)