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Table 6 PCR Master Mix components utilized in PCR prior to 16 S rRNA gene sequencing

From: Alterations to the bovine bacterial ocular surface microbiome in the context of infectious bovine keratoconjunctivitis

AccuPrime â„¢ Taq DNA Polymerase System

10X AccuPrime™ PCR Buffer II (500 μL) containing:

 • 200 mM Tris-HCl (pH 8.4)

 • 500 mM KCl

 • 15 mM MgCl2

 • 2 mM dGTP

 • 2 mM dATP

 • 2 mM dTTP

 • 2 mM dCTP

 • Thermostable AccuPrime™ protein

 • 10% glycerol

 • 50 mM Magnesium Chloride (500 μl)

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Animal Microbiome

ISSN: 2524-4671