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Table 7 Cycling process utilized in PCR prior to 16 S rRNA gene sequencing. The total reaction volume used was 20μL with 20ng of sample DNA

From: Alterations to the bovine bacterial ocular surface microbiome in the context of infectious bovine keratoconjunctivitis

Run Stage

Temperature

Time

Step 1

95℃

3 min

Step 2: (25 cycles)

95℃

30 s

Step 3

55℃

30 s

Step 4

72℃

30 s

Step 5

72℃

5 min

Step 6: Hold

4℃

 
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Animal Microbiome

ISSN: 2524-4671