Table 8 A novel RT-PCR primer panel for detection of IBK. Normalization was performed using the ‘universal bacteria’ and Escherichia coli primer sets
Target | Forward primer | Reverse primer | Reference |
|---|---|---|---|
Bovine GAPDH | CCTGGAGAAACCTGCCAAGT | GCCAAATTCATTGTCGTACCA | [73] |
Moraxella bovis | GGTGACGACCGCTTGTTT | ATCATCGCCTTCATCTCCAG | [63] |
Moraxella bovoculi | GGTGATATTTATCATGAAGTTGTGAAA | TYTCAATTCATAATCACGATACTCAAG | [63] |
Mycoplasma | TGCACCATCTGTCACTCTGTTAACCTC | ACTCCTACGGGAGGCAGCAGTA | [74] |
Staphylococcus | GGCCGTGTTGAACGTGGTCAAATCA | TIACCATTTCAGTACCTTCTGGTAA | [77] |
Pasteurellaceae | CATAAGATGAGCCCAAG | GTCAGTACATTCCCAAGG | [75] |
Prevotellaceae | GGTTCTGAGAGGAAGGTCCCC | TCCTGCACGCTACTTGGCTG | [76] |
Weeksellaceae | ATCCAGCCATCCCGCGT | CTGCTGGCACGGAGTTAGC | None; novel |
Universal Bacteria | CCTACGGGAGGCAGCAGT | ATTACCGCGGCTGCTGG | [35] |
Escherichia coli | CCGATACGCTGCCAATCAGT | ACGCAGACCGTAGGCCAGAT | [78] |