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Table 9 PCR FastMix components utilized in RT-PCR

From: Alterations to the bovine bacterial ocular surface microbiome in the context of infectious bovine keratoconjunctivitis

PerfecCTa SYBR® Green FastMix

 • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, and dTTP

 • AccuStart II Taq DNA Polymerase

 • SYBR Green I dye

 • Proprietary enzyme stabilizers and performance-enhancing additives