Dietary novel alkaline protease from Bacillus licheniformis improves broiler meat nutritional value and modulates intestinal microbiota and metabolites

Table 2 Effects of novel protease on fat content and fatty acid profiles in breast muscle of broilers

Items	Protease (g/t)					SEM	P value	L	Q
Items	0	100	200	300	400	SEM	A	L	Q
C8:0, g/100g	0.0106^b	0.0114^b	0.0214^a	0.0090^b	0.0087^b	0.001	0.001	0.482	0.031
C14:0, g/100g	0.0116	0.0101	0.0099	0.0077	0.0081	0.001	0.238	0.024	0.077
C16:0, g/100g	0.4728	0.4640	0.4670	0.3385	0.3692	0.024	0.220	0.045	0.134
C16:1, g/100g	0.0764	0.0736	0.0601	0.0542	0.0462	0.005	0.368	0.036	0.115
C18:0, g/100g	0.1775	0.1862	0.2210	0.1355	0.1717	0.010	0.077	0.371	0.492
C18:1n9, g/100g	0.6265	0.6270	0.5577	0.4248	0.4287	0.036	0.177	0.016	0.058
C18:2n6, g/100g	0.3305	0.3377	0.3173	0.2367	0.2657	0.018	0.313	0.068	0.194
C18:3n3, g/100g	0.0147	0.0152	0.0133	0.0099	0.0105	0.001	0.291	0.041	0.127
C20:1, g/100g	0.0078	0.0077	0.0067	0.0054	0.0060	< 0.001	0.170	0.021	0.069
C20:2, g/100g	0.0081	0.0081	0.0073	0.0070	0.0087	< 0.001	0.741	0.953	0.566
C20:3n6, g/100g	0.0154	0.0171	0.0148	0.0134	0.0182	0.001	0.366	0.753	0.550
C22:1n9, g/100g	0.0071^b	0.0111^b	0.0161^a	0.0080^b	0.0109^b	0.001	< 0.001	0.423	0.043
C22:6n3, g/100g	0.0060	0.0058	0.0066	0.0053	0.0076	< 0.001	0.379	0.296	0.407
SFA, g/100g	0.6725	0.6717	0.7193	0.4907	0.5577	0.033	0.165	0.082	0.198
MUFA, g/100g	0.7178	0.7193	0.6406	0.4924	0.4917	0.042	0.196	0.018	0.063
PUFA, g/100g	0.3747	0.3838	0.3593	0.2722	0.3107	0.020	0.349	0.087	0.238
n-3 PUFA, g/100g	0.0207	0.0210	0.0199	0.0152	0.0181	0.001	0.510	0.182	0.412
n-6 PUFA, g/100g	0.3459	0.3547	0.3321	0.2500	0.2839	0.018	0.330	0.079	0.219
PUFA: SFA, g/100g	0.5589^a	0.5675^a	0.4910^b	0.5568^a	0.5536^a	0.009	0.047	0.749	0.300
n-6: n-3, g/100g	16.7626	17.0661	16.6527	16.3823	15.8423	0.224	0.519	0.112	0.204
TFAC, g/100g	1.77	1.78	1.73	1.25	1.36	0.094	0.223	0.042	0.126
IMF, g/100g	1.93	1.95	1.95	1.37	1.52	0.105	0.223	0.055	0.153

Results are the means of each group of 6 broilers. SFA, saturated fatty acids; MUFA, monounsaturated fatty acids; PUFA, polyunsaturated fatty acids; TFAC, total fatty acids; IMF, intramuscular fat; A, ANOVA; L, linear; Q, quadratic
^a⁻^bMeans within a row with different superscripts are significantly different (P < 0.05)

ISSN: 2524-4671