Dynamic effects of black soldier fly larvae meal on the cecal bacterial microbiota and prevalence of selected antimicrobial resistant determinants in broiler chickens

Table 1 Multiple pairwise comparisons of the cecal bacterial microbiota between broiler chickens fed varied amounts of BSFLM and a conventional soybean-based diet with or without BMD

		Pairwise comparison statistics²
Day	Diet Comparisons¹	R² value	Adjusted p value³
14
	BMD vs. 0% BSFLM	0.0462	1.000
	BMD vs. 12.5% BSFLM	0.0394	1.000
	BMD vs. 25% BSFLM	0.0539	1.000
	BMD vs. 50% BSFLM	0.0398	1.000
	BMD vs. 100% BSFLM	0.1402	0.015
	0% vs. 12.5% BSFLM	0.0377	1.000
	0% vs. 25% BSFLM	0.0835	0.270
	0% vs. 50% BSFLM	0.0651	1.000
	0% vs. 100% BSFLM	0.189	0.015
	12.5% vs. 25% BSFLM	0.0379	1.000
	12.5% vs. 50% BSFLM	0.0366	1.000
	12.5% vs. 100% BSFLM	0.1365	0.030
	25% vs. 50% BSFLM	0.0227	1.000
	25% vs. 100% BSFLM	0.0888	0.180
	50% vs. 100% BSFLM	0.0682	0.840
28
	BMD vs. 0% BSFLM	0.1446	0.015
	BMD vs. 12.5% BSFLM	0.0472	1.000
	BMD vs. 25% BSFLM	0.0293	1.000
	BMD vs. 50% BSFLM	0.0827	0.045
	BMD vs. 100% BSFLM	0.1243	0.015
	0% vs. 12.5% BSFLM	0.1509	0.015
	0% vs. 25% BSFLM	0.1358	0.015
	0% vs. 50% BSFLM	0.2988	0.015
	0% vs. 100% BSFLM	0.3445	0.015
	12.5% vs. 25% BSFLM	0.0473	1.000
	12.5% vs. 50% BSFLM	0.1312	0.015
	12.5% vs. 100% BSFLM	0.1862	0.015
	25% vs. 50% BSFLM	0.0809	0.030
	25% vs. 100% BSFLM	0.1270	0.015
	50% vs. 100% BSFLM	0.0823	0.075
35
	BMD vs. 0% BSFLM	0.0581	1.000
	BMD vs. 12.5% BSFLM	0.0810	0.300
	BMD vs. 25% BSFLM	0.0558	1.000
	BMD vs. 50% BSFLM	0.1225	0.030
	BMD vs. 100% BSFLM	0.2998	0.015
	0% vs. 12.5% BSFLM	0.0509	1.0000
	0% vs. 25% BSFLM	0.0609	0.855
	0% vs. 50% BSFLM	0.1680	0.015
	0% vs. 100% BSFLM	0.3223	0.015
	12.5% vs. 25% BSFLM	0.0320	1.0000
	12.5% vs. 50% BSFLM	0.1008	0.075
	12.5% vs. 100% BSFLM	0.2665	0.015
	25% vs. 50% BSFLM	0.0971	0.060
	25% vs. 100% BSFLM	0.2744	0.015
	50% vs. 100% BSFLM	0.1459	0.015

¹ BMD, bacitracin methylene disalicylate control; BSFLM, black soldier fly meal; %, percentage of soybean meal replaced
with black soldier fly larvae meal (BSFLM)
²Error-corrected multiple pairwise comparison was performed using R package pairwiseAdonis with Bonferroni correction
and 999 permutations
³Significant differences are bolded (p < 0.05)

ISSN: 2524-4671