Dynamic effects of black soldier fly larvae meal on the cecal bacterial microbiota and prevalence of selected antimicrobial resistant determinants in broiler chickens

Lau, Calvin Ho-Fung; Capitani, Sabrina; Tien, Yuan-Ching; Verellen, Lou Ann; Kithama, Munene; Kang, Hellen; Kiarie, Elijah G.; Topp, Edward; Diarra, Moussa S.; Fruci, Michael

doi:10.1186/s42523-024-00293-9

Animal Microbiome

Table 2 The abundance of selected antimicrobial resistance determinants in day 35 chicken litter and cecal samples of birds fed BMD, 0%, 12.5%, and 25% BSFLM

From: Dynamic effects of black soldier fly larvae meal on the cecal bacterial microbiota and prevalence of selected antimicrobial resistant determinants in broiler chickens

Target gene copy number/rrnS gene copy number (x 10^− 6) ± SEM²
Diet¹	Litter				Cecum
Diet¹	BMD	0%	12.5%	25%	BMD	0%	12.5%	25%
Gene Target
strA	154026 ± 80294	288021 ± 127212	448690 ± 193791	199109 ± 68265	357 ± 231	5445 ± 3377	28210 ± 26643	2219 ± 1682
strB	24579 ± 12467	45758 ± 20377	78221 ± 40171	31973 ± 12834	79.9 ± 49.24	1432 ± 870	6896 ± 6428	531 ± 373
tetA	81110 ± 25549	219589 ± 88024	153168 ± 65460	104049 ± 22118	2058 ± 411	5984 ± 1733	1724 ± 419	2005 ± 935
blaCMY-2	24927 ± 20655	7491 ± 2646	44986 ± 20659	10421 ± 4924	32.6 ± 16.96	11.2 ± 4.12	38.6 ± 11.17	14.1 ± 6.59
sul1	3499 ± 2059	6692 ± 3563	3410 ± 2362	5248 ± 1416	37.1 ± 31.87	347 ± 313	49.5 ± 38.68	19.0 ± 11.79
sul2	20400 ± 11986	12249 ± 4469	26566 ± 10196	23327 ± 9266	5.63 ± 2.70	564 ± 555	83.2 ± 47.23	3.74 ± 3.20
ermF	0.228 ± 0.121	0.128 ± 0.031	0.146 ± 0.039	0.128 ± 0.022	BDL	BDL	BDL	BDL
mphE	0.228 ± 0.12	0.140 ± 0.037	1.10 ± 0.957	0.128 ± 0.022	BDL	BDL	BDL	BDL
Int1	23599 ± 11567	21405 ± 6644	22718 ± 8062	25089 ± 8320	92.64 ± 71.20	2725.±2595	373 ± 177	57.1 ± 31.22
blaOXA-20	0.697 ± 0.540	336 ± 335	0.801 ± 0.535	0.709 ± 0.264	0.501 ± 0.123	0.384 ± 0.084	1.72 ± 0.926	0.351 ± 0.073
msrE	0.228 ± 0.121	0.464 ± 0.235	8.5 ± 8.35	0.141 ± 0.025	BDL	BDL	BDL	BDL
ermB	5310 ± 2697	16431 ± 6966	6944 ± 2711	2783 ± 816	191787 ± 29057	104689 ± 22512^*	93115 ± 15602^*	64585 ± 9922^**
aadA	1578 ± 974	2705 ± 1465	1732 ± 0.52	2498 ± 617	27.2 ± 23.36	323 ± 291	42.8 ± 28.77	16.7 ± 10.25
bcrR	15954 ± 6021	4660 ± 1173	8188 ± 3583	6015 ± 1485	11465 ± 3494	2385 ± 893^*	2004 ± 1051^**	1218 ± 648^**

¹ BMD, bacitracin methylene disalicylate control; BSFLM, black soldier fly meal; %, percentage of soybean meal replaced with black soldier fly larvae meal (BSFLM)
² Values are presented as means ± standard error of the mean of 8 biological replicates. Non-parametric Mann-Whitney U tests were conducted to assess significant differences between the BMD group and the 0%, 12.5%, or 25% BSFLM groups for each antibiotic resistance determinant. Significant differences are indicated in bold, and p-values are denoted as follows: *, p < 0.05, ** p < 0. 005. BDL, below detection limit

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ISSN: 2524-4671

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