Table 2 Effects of hay quality and concentrate supplementation on alpha diversity indices along the gastrointestinal tract of calves

Item

MQH¹

HQH²

MQH + C³

HQH + C⁴

SEM⁵

Hay quality

Concentrate

Hay quality × Concentrate

Reticulum

Observed sOTU⁶

1109^a

861^b

500^c

502^c

51.3

0.02

< 0.01

0.02

Shannon

5.31

4.80

3.27

3.23

0.14

0.05

< 0.01

0.10

InvSimpson

57.8^a

33.1^b

11.5^c

9.33^c

4.94

0.01

< 0.01

0.03

Fisher’s

238^a

171^b

84.4^c

83.6^c

13.0

0.02

< 0.01

0.02

Rumen liquid

Observed sOTU

1051

895

570

522

74.0

0.15

< 0.01

0.44

Shannon

4.99

4.80

3.33

3.21

0.17

0.31

< 0.01

0.81

InvSimpson

33.4

32.1

11.7

9.72

5.27

0.74

< 0.01

0.94

Fisher’s

221

178

98.2

89.8

17.4

0.13

< 0.01

0.30

Rumen solid

Observed sOTU

1059

880

599

550

74.0

0.12

< 0.01

0.36

Shannon

5.33

4.88

3.37

3.33

0.15

0.09

< 0.01

0.16

InvSimpson

65.1^a

39.4^b

10.9^c

10.4^c

3.47

< 0.01

< 0.01

< 0.01

Fisher’s

226

174

106

98.6

18.0

0.09

< 0.01

0.20

Abomasum

Observed sOTU

1078

895

659

605

80.2

0.16

< 0.01

0.43

Shannon

4.95

4.80

3.42

3.46

0.21

0.81

< 0.01

0.66

InvSimpson

43.8

33.5

10.8

11.4

6.05

0.44

< 0.01

0.38

Fisher’s

220

178

120

107

18.3

0.15

< 0.01

0.45

Duodenum

Observed sOTU

1062^a

881^b

521^c

672^c

55.4

0.79

< 0.01

0.01

Shannon

5.15

4.67

3.30

3.42

0.20

0.37

< 0.01

0.15

InvSimpson

45.4

31.7

11.4

10.7

18.7

0.33

< 0.01

0.77

Fisher’s

215

176

88.8

119

13.8

0.76

< 0.01

0.20

Jejunum

Observed sOTU

1058

896

581

588

73.3

0.31

< 0.01

0.27

Shannon

5.09

4.67

2.64

3.09

0.27

0.96

< 0.01

0.13

InvSimpson

75.2

28.6

5.81

9.00

25.7

0.41

0.10

0.35

Fisher’s

217

175

97.5

99.7

32.6

0.41

< 0.01

0.33

Colon

Observed sOTU

1225

980

617

639

72.4

0.14

< 0.01

0.08

Shannon

5.70

5.33

4.21

4.22

0.17

0.31

< 0.01

0.28

InvSimpson

93.8

65.4

25.6

27.6

13.5

0.34

< 0.01

0.28

Fisher’s

279^a

204^b

112^c

117^c

17.5

0.06

< 0.01

0.04