Fig. 1

Average of percentage of reads from ITS2 sequencing: Average of percentage of ITS2 sequencing reads from Ae. aegypti, Ae. albopictus and Cx. quinquefasciatus. Ae. aegypti samples were sequencing again with the addition of a PNA blocker targeting the host ITS sequence. To generate average reads per species 11 laboratory reared and 22 field collected samples from Ae. albopictus and Cx. quinquefasciatus were analysed to generate average reads per species. For Ae. aegypti 22 field collected samples were assessed while 16 field collected samples were amplified with the PNA blocker