Fig. 6

Fungal colonization in presence or absence of a native microbiome. R. mucilaginosa, C. oliophila, L. thermotolerans were incolulated into conventionally (C) reared Ae. aegypti mosquitoes that possessed their native microbiota or axenic germ-free mosquitoes to create a mono-association (MA). CFUs were quantified in A L2-L3 larvae and B three to four day old adults. The bacterium C. neteri was used as a positive control. A contamination control was undertaken by rearing axenic larvae without infection. These mosquitoes did not develop confirming sterility. The CFU/mosquito data were analysed by unpaired t test and prevalence data by a Fishers exact test. Asterisks (*) indicates significance, while ns denotes non-significant