Skip to main content
Fig. 2 | Animal Microbiome

Fig. 2

From: Dietary L-Methionine modulates the gut microbiota and improves the expression of tight junctions in an in vitro model of the chicken gastrointestinal tract

Fig. 2

Methionine supplementation-induced microbial changes of broiler digesta. To confirm the practical application of methionine, the small intestine digest of healthy broilers was co-cultured with methionine using FIMM (in vitro microbial culture) and the intestinal microflora was analyzed. (A) The digesta pH changes were measured during incubation. (B) The richness of the digesta microbiota was assessed through alpha-diversity metrics, specifically employing Chao and Shannon indices. The results are presented as the mean ± SD. (C) To compare the diversity of the digesta microbiota across different groups, PCoA plots were utilized, grounded in both weighted and unweighted UniFrac distances pertaining to the microbiome of broilers digesta. Each plot symbolizes an individual replicate, with the axes depicting the two principal dimensions that capture the most significant variance within the microbial communities. (D) Compositional changes were analyzed under phylum, family, genus, and species level

Back to article page

Animal Microbiome

ISSN: 2524-4671